Objective—To determine the effects of raw meat–based diets with and without inulin or yeast cell-wall (YCW) extract on macronutrient digestibility, blood cell counts, serum metabolite concentrations, and fecal fermentative end-product concentrations in healthy adult dogs.
Procedures—Dogs were fed each of the following 6 diets for 21 days, the order of which was randomly assigned in a Latin square design: beef control, beef and 1.4% inulin, beef and 1.4% YCW extract, chicken control, chicken and 1.4% inulin, and chicken and 1.4% YCW extract. Each diet trial consisted of a phase for diet adaptation (days 0 to 14) and a phase for measurement of urine and fecal output and content (days 15 to 20). On day 21, food was withheld for blood sample collection. Afterward, the next diet trial began immediately.
Results—All dogs maintained desirable fecal quality characteristics and produced low fecal volume. All diets were highly digestible (protein digestibility > 88%; fat digestibility > 97%). Differences in fermentative end-product concentrations among all diets were minor, but a significant increase in fecal short-chain fatty acid concentrations was evident when dogs were fed beef-based diets with inulin and YCW extract. Fecal spermine concentrations were higher with diets containing inulin and YCW extract than with control diets. Blood cell counts and serum metabolite values were within reference limits after each trial. All diets resulted in maintenance of nitrogen balance.
Conclusions and Clinical Relevance—Results suggested the raw meat–based diets evaluated were highly digestible in dogs. The increase in fecal short-chain fatty acid concentrations achieved when inulin and YCW extract were included may be beneficial to canine health.
OBJECTIVE To evaluate canine erythrocyte concentrates (ECs) for the presence of procoagulant phospholipid (PPL), determine whether PPL concentration changes during the course of storage of ECs, and ascertain whether prestorage leukoreduction (removal of leukocytes via gravity filtration) reduces the development of PPL.
SAMPLE 10 whole blood units (420 g each) collected from 10 random-source, clinically normal dogs (1 U/dog).
PROCEDURES The dogs were randomized to 1 of 2 groups. Of the 10 whole blood units collected, 5 were processed through a standard method, and 5 underwent leukoreduction. Whole blood units were processed to generate ECs, from which aliquots were aseptically collected from each unit weekly for 5 weeks. Supernatants from the concentrates were evaluated for procoagulant activity, which was converted to PPL concentration, by use of an automated assay and by measurement of real-time thrombin generation.
RESULTS Supernatants from stored canine ECs contained procoagulant activity as measured by both assays. In general, the PPL concentration gradually increased during the storage period, but leukoreduction reduced the development of increased procoagulant activity over time.
CONCLUSIONS AND CLINICAL RELEVANCE The presence of PPL in canine ECs may be associated with procoagulant and proinflammatory effects in vivo, which could have adverse consequences for dogs treated with ECs.