Objective—To compare blood biochemical values obtained from a handheld analyzer, 2 tabletop analyzers, and 2 diagnostic laboratories by use of replicate samples of sea turtle blood.
Animals—22 captive juvenile sea turtles.
Procedures—Sea turtles (18 loggerhead turtles [Caretta caretta], 3 green turtles [Chelonia mydas], and 1 Kemp's ridley turtle [Lepidochelys kempii]) were manually restrained, and a single blood sample was obtained from each turtle and divided for analysis by use of the 5 analyzers. Hematocrit and concentrations or activities of aspartate aminotransferase, creatine kinase, glucose, total protein, albumin, BUN, uric acid, P, Ca, K, Na, Cl, lactate dehydrogenase, and alkaline phosphatase were determined. Median values for each analyte were compared among the analyzers.
Results—Significant differences were found among the analyzers for most values; however, data obtained from the 2 diagnostic laboratories were similar for all analytes. The magnitude of difference between the diagnostic laboratories and in-house units was ≥ 10% for 10 of the 15 analytes.
Conclusions and Clinical Relevance—Variance in the results could be attributed in part to differences in analyzer methodology. It is important to identify the specific methodology used when reporting and interpreting biochemical data. Depending on the variable and specific case, this magnitude of difference could conceivably influence patient management.
Objective—To determine safety and efficacy of an
anesthetic protocol incorporating medetomidine, ketamine,
and sevoflurane for anesthesia of injured loggerhead
Animals—13 loggerhead sea turtles.
Procedure—Anesthesia was induced with medetomidine
(50 µg/kg [22.7 µg/lb], IV) and ketamine (5
mg/kg [2.3 mg/lb], IV) and maintained with sevoflurane
(0.5 to 2.5%) in oxygen. Sevoflurane was delivered
with a pressure-limited intermittent-flow ventilator.
Heart rate and rhythm, end-tidal partial pressure
of CO2, and cloacal temperature were monitored continuously;
venous blood gas analyses were performed
intermittently. Administration of sevoflurane
was discontinued 30 to 60 minutes prior to the end of
the surgical procedure. Atipamezole (0.25 mg/kg
[0.11 mg/lb], IV) was administered at the end of
Results—Median induction time was 11 minutes
(range, 2 to 40 minutes; n = 11). Median delivered
sevoflurane concentrations 15, 30, 60, and 120 minutes
after intubation were 2.5 (n = 12), 1.5 (12), 1.25
(12), and 0.5% (8), respectively. Heart rate decreased
during surgery to a median value of 15 beats/min (n =
11). End-tidal partial pressure of CO2 ranged from 2 to
16 mm Hg (n = 8); median blood gas values were
within reference limits. Median time from atipamezole
administration to extubation was 14 minutes
(range, 2 to 84 minutes; n = 7).
Conclusions and Clinical Relevance—Results suggest
that a combination of medetomidine and ketamine
for induction and sevoflurane for maintenance
provides safe, effective, controllable anesthesia in
injured loggerhead sea turtles. (J Am Vet Med Assoc