Objective—To compare blood glucose concentrations
obtained using a point-of-care (POC) analyzer, 5 portable
blood glucose meters (PBGM), and a color reagent test
strip with concentrations obtained using a reference
method, and to compare glucose concentrations
obtained using fresh blood samples in the PBGM with
concentrations obtained using blood anticoagulated
with lithium heparin.
Sample Population—110 blood samples from 34
dogs; glucose concentration of the samples ranged
from 41 to 596 mg/dl.
Procedure—Logistic regression was used to compare
blood glucose concentrations obtained with the
various devices with reference method concentrations.
Ease of use was evaluated subjectively.
Percentage of times a clinical decision would have
been altered if results of each of these methods had
been used, rather than results of the reference
method, was calculated.
Results—For 3 of the PBGM, blood glucose concentrations
obtained with fresh blood were not significantly
different from concentrations obtained with
blood samples anticoagulated with lithium heparin.
None of the devices provided results statistically
equivalent to results of the reference method, but the
POC analyzer was more accurate than the others. For
some samples, reliance on results of the PBGM or
the color test strip would have resulted in erroneous
Conclusions and Clinical Relevance—Although
commercially available PBGM and color test strips
provided blood glucose concentrations reasonably
close to those obtained with reference methods,
some devices were more accurate than others. Use
of results from these devices could lead to erroneous
clinical decisions in some cases. ( J Am Vet Med
Objective—To determine the association between
cancer chemotherapy and serum canine distemper
virus (CDV), canine parvovirus (CPV), and rabies virus
antibody titers in tumor-bearing dogs.
Animals—21 client-owned dogs with various malignancies
and 16 client-owned dogs with lymphoma.
Procedure—In study A, serum antibody titers were
measured by use of hemagglutination inhibition (CPV
titers) or serum neutralization (CDV titers) before and
at least 1 month after initiation of chemotherapy.
Baseline values were compared with values obtained
from a control population of 122 healthy dogs seen for
routine revaccination. Titers were considered protective
at ≥ 1:96 for CDV and ≥ 1:80 for CPV.
In study B, serum IgG titers were measured by
use of immunofluorescent assay (CDV and CPV titers)
and rapid fluorescent focus inhibition test (RFFIT,
rabies titers) at baseline and again at weeks 5, 8, and
24 of a standard chemotherapy protocol for treatment
of lymphoma. An IgG titer of ≥ 1:50 was considered
protective for CPV and CDV. An RFFIT titer of ≥ 0.5
U/ml was considered protective for rabies virus.
Results—Significant changes were not detected in
CDV, CPV, and rabies virus titers following chemotherapy
in tumor-bearing dogs.
Conclusions and Clinical Relevance—Results suggest
that established immunity to CDV, CPV, and
rabies virus from previous vaccination is not significantly
compromised by standard chemotherapy used
to treat tumor-bearing dogs. (J Am Vet Med Assoc
OBJECTIVE To conduct a phase I-II clinical trial of hyaluronan-cisplatin nanoconjugate (HA-Pt) in dogs with naturally occurring malignant tumors.
ANIMALS 18 healthy rats, 9 healthy mice, and 16 dogs with cancer.
PROCEDURES HA-Pt was prepared and tested by inductively coupled plasma mass spectrometry; DNA-platinum adduct formation and antiproliferation effects of cisplatin and HA-Pt were compared in vitro. Effects of cisplatin (IV) and HA-Pt (SC) in rodents were tested by clinicopathologic assays. In the clinical trial, dogs with cancer received 1 to 4 injections of HA-Pt (10 to 30 mg/m2, intratumoral or peritumoral, q 3 wk). Blood samples were collected for pharmacokinetic analysis; CBC, serum BUN and creatinine concentration measurement, and urinalysis were conducted before and 1 week after each treatment. Some dogs underwent hepatic enzyme testing. Tumors were measured before the first treatment and 3 weeks after each treatment to assess response.
RESULTS No adverse drug effects were detected in pretrial assessments in rodents. Seven of 16 dogs completed the study; 3 had complete tumor responses, 3 had stable disease, and 1 had progressive disease. Three of 7 dogs with oral and nasal squamous cell carcinoma (SCC) that completed the study had complete responses. Myelosuppression and cardiotoxicosis were identified in 6 and 2 dogs, respectively; none had nephrotoxicosis. Four of 5 dogs with hepatic enzymes assessed had increased ALT activities, attributed to diaquated cisplatin products in the HA-Pt. Pharmacokinetic data fit a 3-compartment model.
CONCLUSIONS AND CLINICAL RELEVANCE HA-Pt treatment resulted in positive tumor responses in some dogs, primarily those with SCC. The adverse effect rate was high.
IMPACT FOR HUMAN MEDICINE Oral SCC in dogs has characteristics similar to human head and neck SCC; these results could be useful in developing human treatments.