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microscopy. Approximately 0.1 g of feces was suspended in 10 mL of PBS solution (pH, 7). A 100-μL aliquot of this solution (ie, 0.001 g of feces) was inoculated into a culture tube containing 10 mL of antibiotic-fortified (10 6 U of penicillin, 1.5 g of

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in American Journal of Veterinary Research

differentiation medium (Dulbecco modified Eagle medium containing 2% horse serum, s penicillin-streptomycin [1 U/mL and 1 μg/mL, respectively], and 2mM L-glutamine). Medium was changed approximately every 4 days, and cells were examined daily. Coverslips were

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in American Journal of Veterinary Research

cell culture media, and cultured in DMEM-sup, c which was supplemented with 10% deactivated fetal bovine serum, d 50 μg of penicillin d /mL, 50 μg of streptomycin d /mL, and 29.2 mg of l-glutamine d /mL in a sterile culture incubator at 37°C at 5% CO

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in American Journal of Veterinary Research