Objective—To evaluate the effect of multiple hydrogen peroxide gas plasma (HPGP) sterilizations on the rate of closure of ameroid constrictors.
Sample—Thirty-six 5.0-mm ameroid constrictors.
Procedures—Ameroid constrictors were randomly allocated to 6 groups. Each group underwent 1, 2, 3, 4, 5, or 6 HPGP sterilizations. Ameroid constrictors were then incubated for 35 days in canine plasma and digitally imaged at predetermined times during incubation. One individual, who was unaware of the group to which each ameroid constrictor was assigned, measured the lumen area of the constrictor on each digital image. Mean lumen area was compared among groups.
Results—No ameroid constrictors were completely closed after 35 days of incubation in canine plasma. Mean lumen area after incubation did not differ among constrictors that underwent 1, 2, and 3 sterilizations. Constrictors that underwent 4 sterilizations were closed significantly more than were those that underwent 1, 2, or 3 sterilizations. Mean lumen area after incubation did not differ significantly between constrictors that underwent 5 and 6 sterilizations, although the final lumen areas for those constrictors were significantly smaller than those for constrictors that underwent 1, 2, 3, and 4 sterilizations.
Conclusions and Clinical Relevance—Ameroid constrictors that underwent 5 and 6 HPGP sterilizations had a 9% to 12% decrease in lumen area, compared with that of constrictors that underwent ≤ 4 plasma sterilizations, and the use of such constrictors could increase the risk of portal hypertension and secondary acquired shunting or decrease the risk of persistent shunting.
Objective—To develop a reverse transcriptase-polymerase
chain reaction (RT-PCR) assay to detect feline
herpesvirus-1 (FHV-1) latency-associated transcripts
(LATs) in the corneas and trigeminal ganglia of cats
that did not have clinical signs of ocular disease.
Sample Population—Corneas and trigeminal ganglia
obtained from 21 cats necropsied at the Indiana
Animal Disease Diagnostic Laboratory and 25 cats
euthanatized at a humane shelter; none of the cats
had a recent history of respiratory tract or ocular disease,
and all had normal results for ophthalmic examinations.
Procedure—Both corneas and both trigeminal ganglia
were harvested from each cat. An initial PCR assay
detected FHV-1 DNA in the corneas and trigeminal
ganglia. The RNA was then isolated from samples
positive for FHV-1 DNA, and an RT-PCR assay was
used to detect LATs.
Results—FHV-1 DNA was detected in 45 of 92
(48.9%) corneas and 38 of 92 (41.3%) trigeminal ganglia.
In many samples, the RNA had degraded and RTPCR
assay was not possible. Of the samples subjected
to RT-PCR assay, none of the 39 corneas but 4 of
16 trigeminal ganglia had positive results when tested
Conclusions and Clinical Relevance—Analysis of
the results indicated that a high percentage of cats
that did not have clinical signs of ocular disease had
detectable FHV-1 DNA in their corneas and trigeminal
ganglia. This study documents that the RT-PCR assay
can successfully identify LATs and may serve as a
tool to better understand the biologic characteristics
of FHV-1 and its relationship to clinical disease. ( Am J Vet Res 2004;65:314–319)