Objective—To evaluate the clinical and analytic accuracy
of 5 portable blood glucose meters (PBGM) in
cats, with emphasis on the detection of potential
sources of error.
Procedure—Venous blood glucose readings from 5
PBGM were compared with the results of a hexokinase
reference method. Agreement among methods
was determined by error grid analysis and statistical
Results—A total of 2,975 PBGM readings and 513 reference
values were analyzed. The accuracy of the
PBGM varied in different glycemic ranges. The largest
differences between PBGM readings and reference values
were in the high glycemic range; 4 PBGM underestimated
and 1 PBGM overestimated the reference
values in most instances. In the low and reference
glycemic ranges, the absolute differences between
PBGM readings and reference values were small.
Despite the analytic differences in accuracy, 4 PBGM
had 100% and 1 PBGM had 98.7% of readings in the
clinically acceptable values of the error grid analysis.
Within- and between-day precisions were good for all
PBGM. Significant differences were not detected
between readings of EDTA and lithium-heparinized
blood and fresh blood without anticoagulant. Compared
with these blood types, 1 PBGM had significantly different
readings with fluoride anticoagulated blood. In
blood samples with a low Hct, all PBGM overestimated
glucose concentrations. Sample volumes < 3 µl resulted
in inaccurate measurements in 3 PBGM.
Conclusions and Clinical Relevance—Performance
varied among the 5 PBGM analyzed; however, all
PBGM were deemed acceptable for clinical use in
cats. (Am J Vet Res 2000;61:1587–1592)
Objective—To evaluate the diagnostic value of plasma N-terminal pro-B-type natriuretic peptide (NT-proBNP) concentrations in Doberman Pinschers in various stages of dilated cardiomyopathy (DCM).
Animals—328 Doberman Pinschers.
Procedures—Staging of DCM was determined via analysis of results of physical examinations, 24-hour ambulatory ECG (Holter) recordings, and echocardiographic evaluations. Plasma samples for NT-proBNP assays were obtained at each examination. Concentrations of NT-proBNP were measured in 337 samples obtained from 196 healthy Doberman Pinschers (control dogs) and in 195 samples obtained from 132 Doberman Pinschers in various stages of DCM. These included dogs that had ventricular premature contractions (VPCs; 79 samples), echocardiographic changes (23 samples), or both (51 samples); 16 samples were from dogs with overt DCM, and 26 were from dogs that were considered normal during initial examination but developed DCM within 1.5 years after this assessment. Receiver operating characteristic curves were analyzed to determine sensitivity and specificity of NT-proBNP concentrations for detection of DCM.
Results—NT-proBNP concentrations in dogs that had or developed DCM were significantly higher than those of control dogs. Sensitivity and specificity of NT-proBNP concentrations (cutoff value, > 400 pmol/L) to detect all stages of DCM were 81.1 % and 75.0%, respectively; sensitivity was 90.0% and specificity was 75.0% to predict echocardiographic changes. Specificity to detect echocardiographic changes was 90.4% at a cutoff value of 550 pmol/L.
Conclusions and Clinical Relevance—Plasma concentrations of NT-proBNP were increased in dogs with DCM and in apparently healthy dogs that developed DCM within 1.5 years after samples were obtained, compared with concentrations in control dogs.