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Summary

A study was designed to compare use of an numerical rating scale (nrs) and a visual analogue scale (vas) for subjective assessment of lameness, using sheep as a model. The nrs consisted of 5 divisions, 0, 1, 2, 3, and 4; 4 of these divisions (1–4) described lameness. The vas used a 100-mm horizontal line with vertical bars at either end; one end was labeled 'sound' and the other was labeled 'could not be more lame.' Two independent observers graded lameness in 62 sheep, and between- and within-observer differences were assessed for each scoring system to compare the nrs with the vas.

Results indicated no significant differences between the 2 observers scoring lameness, using either the vas or the nrs. The scores obtained, using the vas, were not normally distributed, although differences between scores for the 2 observers were. The nrs scores followed a normal distribution pattern. Investigation of repeated measurement for the same sheep, using both scales, revealed no significant difference between either. A comparison of the nrs and vas scores made by each observer indicated that although correlation was good (observer 1; r = 0.94; observer 2; r = 0.95), there was not perfect agreement. The maximal nrs score of 4 was associated with vas values > 68 mm, indicating that the nrs divisions did not reflect equal increases in lameness. The vas and nrs scores for each observer were highly reproducible, although they were more variable for sheep that were regarded as moderately lame.

Results indicate that although the nrs and vas compared favorably with respect to repeatability, reproducibility, and use by 2 observers, the vas is inherently more sensitive. In addition, the nrs and vas should not be used interchangeably.

Free access
in American Journal of Veterinary Research

Abstract

OBJECTIVE

To investigate the time course of circulating neutrophil priming and activity in dogs with spinal cord injury secondary to intervertebral disk herniation that undergo decompressive surgery.

ANIMALS

9 dogs with spinal cord injury and 9 healthy dogs (controls).

PROCEDURES

For dogs with spinal cord injury, blood samples were collected on the day of hospital admission and 3, 7, 30, and 90 days after injury and decompressive surgery. A single blood sample was collected from the control dogs. Flow cytometry analysis was performed on isolated neutrophils incubated with antibody against CD11b and nonfluorescent dihydrorhodamine 123, which was converted to fluorescent rhodamine 123 to measure oxidative burst activity.

RESULTS

Expression of CD11b was increased in dogs with spinal cord injury 3 days after injury and decompressive surgery, relative to day 7 expression. Neutrophils expressed high oxidative burst activity both 3 and 7 days after injury and decompressive surgery, compared with activity in healthy dogs.

CLINICAL RELEVANCE

For dogs with spinal cord injury, high CD11b expression 3 days after injury and decompressive surgery was consistent with findings for rodents with experimentally induced spinal cord injury. However, the high oxidative burst activity 3 and 7 days after injury and decompressive surgery was not consistent with data from other species, and additional studies on inflammatory events in dogs with naturally occurring spinal cord injury are needed.

Full access
in American Journal of Veterinary Research