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  • Author or Editor: Denise A. Elliott x
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Abstract

Objective—To evaluate the use of multifrequency bioelectrical impedance analysis (MF-BIA) for estimating total body water (TBW), extracellular fluid volume (ECFV), and intracellular fluid volume (ICFV) in horses.

Animals—9 healthy mares.

Procedure—TBW and ECFV were measured by use of deuterium oxide and sodium bromide dilution techniques, respectively. Intracellular fluid volume was calculated as the difference between TBW and ECFV. Concurrently, MF-BIA recordings were obtained by use of 4 anatomic electrode positions and 3 measurements of length. Models for MF-BIA data were created for all combinations of length and anatomic electrode position. Models were evaluated to determine the position-length configuration that provided the most consistent estimates of TBW, ECFV, and ICFV, compared with values determined by use of the dilution techniques.

Results—Positioning electrodes over the ipsilateral carpus and tarsus and use of height at the tuber sacrale for length provided the closest estimate between values for TBW, ECFV, and ICFV predicted by use of MF-BIA and measured values obtained by dilutional techniques. This model had the narrowest 95% limits of agreement.

Conclusions and Clinical Relevance—MF-BIA techniques have been used to predict changes in TBW, ECFV, and ICFV in healthy and diseased humans. Results reported in this study provide an equine-specific model to serve as the basis for further evaluation of MF-BIA in horses with altered fluid states. The MF-BIA techniques have a number of potential applications for use in horses, including evaluation of exercise physiology, pharmacologic studies, and critical-care management. ( Am J Vet Res 2004;65:320–326)

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in American Journal of Veterinary Research

Abstract

Objective—To characterize the effect of maintenance hemodialysis on plasma amino acid concentrations and to quantitate free amino acid losses into the dialysate during hemodialysis in healthy dogs.

Animals—8 healthy adult dogs.

Procedure—Five dogs received hemodialysis treatments 3 times per week for 4 weeks. Plasma amino acid concentrations were evaluated once per week for 4 weeks in each of the 5 dogs prior to hemodialysis (time 0), 90 minutes during hemodialysis, and immediately after hemodialysis (180 minutes). Total free amino acid concentrations and plasma amino acid concentrations (time 0, 90 minutes, and 180 minutes) in the dialysate were evaluated in 3 dogs that received 1 hemodialysis treatment.

Results—Significant time versus week interactions with any plasma amino acid were not detected; however, significant decreases in all plasma amino acid concentrations measured were detected at the midpoint of dialysis (46 ± 2%) and at the end of each dialysis session (38 ± 2%). Mean (± SEM) total free amino acid loss into the dialysate was 2.7 ± 0.2 g or 0.12 g/kg of body weight.

Conclusions and Clinical Relevance—Hemodialysis is associated with significant alterations in plasma amino acid concentrations and loss of free amino acids into the dialysate. Loss of amino acids into the dialysate, coupled with protein calorie malnutrition in uremic patients, may contribute to depletion of amino acid stores.(Am J Vet Res 2000;61:869–873)

Full access
in American Journal of Veterinary Research