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Abstract
OBJECTIVE
To examine bicarbonate (HCO3 −) secretion ex vivo in the equine large colon to determine any differences between the right dorsal colon (RDC) and right ventral colon (RVC). The effect of phenylbutazone (PBZ) on HCO3 − secretion was examined in the RDC.
ANIMALS
14 healthy horses.
PROCEDURES
In anesthetized horses (n = 10), segments of mucosa from RDC and RVC were harvested to measure HCO3 − secretion ex vivo with the pH Stat method. The effect of PBZ on HCO3 − secretion in the RDC was studied in 4 additional horses.
RESULTS
Three distinct mechanisms of HCO3 − secretion previously described in a murine model were confirmed in the equine colon. The RDC had a greater capacity for electrogenic, Cl−-independent HCO3 − secretion than the RVC (P = 0.04). In the RDC, all HCO3 − secretion was decreased by PBZ (P < 0.02) but was not studied in the RVC because of low baseline secretion.
CLINICAL RELEVANCE
Secretion of HCO3 − by the RDC could play a pivotal role in equine colon physiology, because intense microbial fermentation in this site could require HCO3 − secretion to buffer short-chain fatty acids. Inhibition of this secretion by PBZ could interfere with mucosal buffering and predispose to changes associated with right dorsal colitis.
