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body temperature was measured; approximately 5 mL of blood was drawn from the brachial wing vein, the birds were then euthanized by CO 2 asphyxiation, and the spleens were collected from all birds. Blood was collected into a serum separator tube and

Open access
in American Journal of Veterinary Research

deformation relative to the surrounding normal tissue; it involves use of a color display and provides qualitative or semiquantitative evaluations. In veterinary medicine, strain imaging has been performed on the liver, spleen, and kidneys of clinically normal

Full access
in American Journal of Veterinary Research

study 25 found alteration of miRNA expression in the splenic tissue among dogs with histologically normal spleens, dogs with hemangiosarcoma, and those with splenic nodular hyperplasia. The objective of the present study was to identify miRNAs that

Full access
in American Journal of Veterinary Research

resonance cholangiopancreatography has been described for 10 cats with cholangitis and pancreatitis. 6 To the authors’ knowledge, diffusion and perfusion of the liver, spleen, and kidneys of cats have not been described. Therefore, the objective of the

Full access
in American Journal of Veterinary Research

SUMMARY

Sonographic and/or anatomic observations were made of the spleen in 27 dogs. Anatomic studies were used to establish precise correlations between the gross anatomic features of the organ and its ultrasonographic image. In 8 anesthetized dogs, ultrasonographic images of the spleen were made in dorsal, transverse, and sagittal planes. When it was incident to the ultrasonic beam, the splenic capsule was represented by a fine echogenic line that defined the boundaries of the organ. The splenic substance had a uniformly mottled echogenicity apart from the anechoic lumen of the splenic venous rami, which were detected at and near the hilus of the spleen. Less regularly, splenic arterial rami were detected at the hilus, but not within the splenic substance. Dorsal and transverse images were made with the ultrasonic transducer perpendicular to the left thoracic and abdominal wall at the 11th intercostal space and caudoventrad to it. Sagittal images were produced with the transducer's face directed craniad, placed parallel to the left lateral abdominal wall, and pushed under the costal arch. The adoption of such an ultrasonographic imaging protocol ensures that all of the spleen is inspected. A definitive opinion can then be given as to whether the spleen is normal or abnormal. Pathologic changes in the spleen must also be differentiated from changes in adjacent organs or structures.

Free access
in American Journal of Veterinary Research

The aim of the study reported here was to investigate the effect of high dietary concentrations of NPP on the intestinal lymphocyte subpopulations and the expression of various cytokines in the cecal tonsil and spleen of laying hens experimentally

Full access
in American Journal of Veterinary Research

Summary

Brain, spleen, and selected lymph nodes from sheep with clinical signs of scrapie were analyzed for presence of proteinase K-resistant protein (PrP-res). Diagnosis of scrapie on the basis of detection of PrP-res was compared with diagnosis on the basis of histologic evaluation of the brain from clinically affected or exposed sheep. Proteinase K-resistant protein was found in every brain that was histologically positive for scrapie, and in addition, was found in the brain of several clinically positive sheep that were not diagnosed as scrapie-positive by histologic evaluation. Proteinase K-resistant protein was also found in 87% of the spleens and lymph nodes from sheep that had PrP-res detected in brain homogenates. Therefore, analysis of sheep brain, spleen, or lymph nodes for PrP-res provided a diagnostic approach that was superior to histologic examination alone for detection of naturally scrapie agent-infected sheep.

Free access
in American Journal of Veterinary Research

-needle aspirate samples from the liver or spleen of lymphoma-affected dogs have not been reported. Investigators of previous studies 24–26 used flow cytometry to evaluate tissue samples obtained from the liver or spleen of dogs via biopsy or necropsy. The

Full access
in American Journal of Veterinary Research

Introduction The spleen in cats is a nonsinusoidal structure that has less storage capacity for large amounts of blood, compared with the spleen in dogs. 1 Physiologic splenomegaly is rare in cats, and severe splenomegaly is usually a

Full access
in American Journal of Veterinary Research

The immune system of birds consists of primary and secondary organs. Primary immune organs include the thymus and bursa of Fabricius, and secondary immune organs include the spleen, Harder's gland, bone marrow, and lymphoid tissue such as mucosa

Full access
in American Journal of Veterinary Research