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(Shapiro-Wilks test), the number of days with any clinical sign (daily clinical score > 0) was compared between groups by use of a Student t test. Otherwise, a nonparametric test was performed. The total number of days during which ponies excreted virus

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in American Journal of Veterinary Research

responses within groups were calculated as SD/mean × 100. The mean number of virus copies in spleen lysates at 4 days after infection was compared among groups with the Student t test. Correlations among E2 protein concentration in the vaccine (ie, vaccine

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in American Journal of Veterinary Research

formats. Appropriate model diagnostics (eg, plots of Studentized residuals vs predicted mean) were used to aid model selection and statistical assumptions. Mixed-model methods were used to analyze data. Values of P < 0.05 were considered significant

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in American Journal of Veterinary Research

logarithmic transformation. In some instances, even after transformation, the normality of titer distributions within groups was questionable. Therefore, for each comparison, both the parametric Student t test and the nonparametric Wilcoxon rank sum (Mann

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in American Journal of Veterinary Research
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in American Journal of Veterinary Research

. The absorbance of all wells was standardized and expressed as a percentage of the positive control sample. Statistical analysis —For the ELISA data, a Student t test was used to compare mean absorbances among ponies and between groups by use of

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in American Journal of Veterinary Research