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mixed-breed dogs that underwent laparotomy as part of a veterinary student surgical laboratory were included in the study. Dogs were obtained from USDA-licensed vendors and were considered healthy on the basis of results of physical examination. Dogs

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in American Journal of Veterinary Research

Animals —Healthy dogs were recruited from among staff and students of the University of Pennsylvania veterinary teaching hospital. Pet owners were informed about procedures, and written consent was obtained. Eligible dogs were required to be healthy as

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in American Journal of Veterinary Research

receptor-positive cells per sample as obtained by immunohistochemical analysis and flow cytometry. The Student t test was used to compare the mean percentage of lymphocytes that expressed LH receptors in nonneoplastic versus neoplastic lymph node

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in American Journal of Veterinary Research

, and serum 17β-estradiol, and 17-HPG concentrations were made between clinically normal and clinically affected ferrets by use of the 2-tailed Student t test. Median androstenedione concentrations were compared by means of the Mann-Whitney U test

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in American Journal of Veterinary Research

adrenal glands were obtained within 10 minutes after healthy dogs used for practice surgeries performed by veterinary students were euthanized. Procedures were performed in accordance with published guidelines and as approved by the Auburn University

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in American Journal of Veterinary Research

; when testing was complete, students and staff of the equine center observed the horses for the remainder of the day. When testing required horses to be in a nonfed state, a previously described feed withholding protocol for the OST 3 was followed, in

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in American Journal of Veterinary Research

was obtained from a dog immediately after that dog had been used in the veterinary student surgery laboratory and euthanized; RNA was collected from the pituitary gland by use of a kit. e Primers used to amplify the ACTH sequence were designed on the

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in American Journal of Veterinary Research

available ad libitum. Animal care was in accordance with the guidelines and directives established by the Animal Welfare Act of Switzerland. In addition, 20 healthy student- and staff-owned large-breed dogs, including 10 males (3 castrated) and 10 females

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in American Journal of Veterinary Research

Student t tests were used to make pairwise comparisons between the respective treatment group means. For each ETR (ETR-A and ETR-B), a 2-way ANOVA was used to assess the effect of treatment group and hoof location (proximal and distal) on the gray value

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in American Journal of Veterinary Research

by logarithmic conversion, the distributions of other variables were considered normal ( P > 0.05 [significance set at a value of P < 0.05]). For each variable, independent Student t tests were used to compare the mean values between affected and

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in American Journal of Veterinary Research