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interactions between rickettsial-infected cells and cells in the spleen were present. Despite the high likelihood that cattle in Europe and western North America could be infected with both A marginale and A phagocytophilum , little is known about how

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in American Journal of Veterinary Research

concentration were calculated by the instrument. Differential cell counts were performed manually on Wright-stained blood smears. During necropsy, swab specimens of the liver, spleen, lungs, and mesenteric lymph nodes were obtained by use of a sterile

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in American Journal of Veterinary Research

pentobarbital i (1 mg/kg, IV). Pigs were euthanatized by administration of pentobarbital (1 mg/kg, IP). The spleen was then removed from each animal and submitted for bacteriologic examinations. In vivo infection experiments involving cytopathic strains

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in American Journal of Veterinary Research

22°C. Tissue samples of the gills, trunk kidneys, and spleen were pooled and stored at −20°C in 70% ethanol for PCR assay. Organs were harvested and immersed in neutral-buffered 10% formalin for subsequent histologic evaluation. Samples of the trunk

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in American Journal of Veterinary Research

; serum was harvested and stored at −70°C. Duplicate samples from the thymus, kidneys, liver, lungs, and spleen were collected; 1 set of samples was stored at −70°C for VI, and the second set of samples was fixed in formalin for histologic examination and

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in American Journal of Veterinary Research

changes in tissue samples —All euthanized calves underwent postmortem examination. From each calf, samples of lymphoid tissues (retropharyngeal, tracheobronchial, mesenteric and ileocecal lymph nodes, pharyngeal and lingual tonsils, thymus, spleen, and

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in American Journal of Veterinary Research

dilution. Whereas tissues (including mesenteric lymph nodes, ovaries, spleen, lungs, placenta, whole fetuses, and fetal skin) were collected from any does that died, a complete necropsy was not performed. In fetuses that yielded negative results for BVDV

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in American Journal of Veterinary Research

-inoculated and control pigs were euthanized, tissue samples (inguinal, axillary, mesenteric, and bronchial lymph nodes; thymus; bone marrow; spleen; liver; lungs; kidneys; and ileum) were collected into cold (4°C) ethanol, fixed for 24 hours, and then processed

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in American Journal of Veterinary Research

, kidneys, spleen, and intestines were immersed in neutral-buffered 10% formalin for histologic evaluation; tissues were processed routinely and embedded in paraffin, sectioned at a thickness of 3 μm, and stained with H&E. For the safety phase, histologic

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in American Journal of Veterinary Research

, results of 1 study 6 suggest that nervous tissue serves as an important niche for uninhibited proliferation of L monocytogenes during infection of a host because the number of L monocytogenes in gerbils decreased in the liver and spleen but not the

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in American Journal of Veterinary Research