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group of reptiles. Therefore, it is imperative that we pursue evidence-based research focused on closing this informational gap to ensure best practices for these animals. Plasma biochemistry testing is commonly performed as a component of baseline

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in American Journal of Veterinary Research

Abstract

Activities of diagnostically important enzymes were measured in serum and lysates of liver, kidney, skeletal muscle, heart, intestine, lung, and pancreatic tissues from wild-caught yellow rat snakes, Elaphe obsoleta quadrivitatta. All samples were analyzed for alkaline phosphatase, lactate dehydrogenase (ld), aspartate transaminase (ast), alanine transaminase, γ-glutamyltransferase, and creatine kinase (ck) activities. The major enzyme activities found in the liver were ld and ast. The kidney had moderate activities of ld, ast, alanine transaminase, and ck. Skeletal muscle and heart contained high ck activity. Intestine, lung, and pancreas had low activities for most enzymes analyzed. Little to no γ-glutamyltransferase activity was found in serum or tissues analyzed. Serum enzyme activities in yellow rat snakes were similar to those described for other reptile species, except for serum ck activity, which was increased in rat snakes.

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in American Journal of Veterinary Research

Abstract

Objective

To identify and characterize blood cells from free-ranging Hawaiian green turtles, Chelonia mydas.

Sample Population

26 green turtles from Puako on the island of Hawaii and Kaneohe Bay on the island of Oahu.

Procedure

Blood was examined, using light and electron microscopy and cytochemical stains that included benzidine peroxidase, chloroacetate esterase, alpha naphthyl butyrate esterase, acid phosphatase, Sudan black B, periodic acid-Schiff, and toluidine blue.

Results

6 types of WBC were identified: lymphocytes, monocytes, thrombocytes, heterophils, basophils, and eosinophils (small and large). Morphologic characteristics of mononuclear cells and most granulocytes were similar to those of cells from other reptiles except that green turtles have both large and small eosinophils.

Conclusions

Our classification of green turtle blood cells clarifies improper nomenclature reported previously and provides a reference for future hematologic studies in this species. (Am J Vet Res 1998;59:1252–1257)

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in American Journal of Veterinary Research

. Samples refrigerated at 4°C were analyzed at 8, 24, and 48 hours, whereas samples frozen at −20°C were analyzed at 48 and 72 hours and 7 days. All analyses were performed by use of a veterinary bench-top analyzer f with a predetermined reptile biochemical

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in American Journal of Veterinary Research

in turtles and other reptiles have been attributed to lymph contamination as a result of the close proximity of lymphatics to blood vasculature. 12,14 In sharks, several sites have been used for antemortem blood collection, including the caudal tail

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in American Journal of Veterinary Research

Veterinarians and Association of Reptile and Amphibian Veterinarians Joint Annual Conference, Dallas, September 2017. ABBREVIATIONS BG Blood glucose cVPBG Veterinary portable blood glucometer on canine setting fVPBG Veterinary portable blood

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in American Journal of Veterinary Research