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Committee. Isolation of platelets —Approximately 100 mL of venous blood was collected into 0.4M EDTA, and erythrocytes were allowed to settle for 30 minutes. The leukocyte-containing, platelet-rich plasma was removed and centrifuged at 350 × g for 10

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in American Journal of Veterinary Research

. Erythrocytes were allowed to sediment for 30 minutes, and the platelet- and leukocyte-rich plasma was collected and centrifuged at 350 × g for 10 minutes at 4°C (22°C for flow cytometric studies). The platelet-rich plasma was collected and centrifuged at 1

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in American Journal of Veterinary Research