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), and platelet activation (CD62 expression) in platelet-rich plasma from Cavalier King Charles Spaniels with various stages of MMVD and assess the influence of thrombocytopenia on those variables. Materials and Methods Animals The study was

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in American Journal of Veterinary Research

administration and are subject to marked variability. 12,29 Platelet function can be assessed in vitro by use of aggregometry with whole blood, platelet-rich plasma, or washed platelets. Light transmission aggregometry following stimulation of platelet-rich

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in American Journal of Veterinary Research

was added; and the platelet-rich plasma was centrifuged at 400 × g for 15 minutes at room temperature. Platelet pellets were then suspended in 10 mL of Tyrode-HEPES (12mM NaHCO 3 , 138mM NaCl, 2.9mM KCl, 10mM HEPES, and 5 μg of PGE 1 /mL; pH, 7

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in American Journal of Veterinary Research

protamine causes increased aggregation of platelets and thrombocytopenia in dogs, 6 and results of an in vitro study 7 indicate protamine decreases adenosine diphosphate–induced platelet aggregation. In platelet-rich plasma, the effects of protamine on

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in American Journal of Veterinary Research

of aggregation IC 50 Concentration of an agent that caused 50% inhibition of aggregation PPP Platelet-poor plasma PRP Platelet-rich plasma a. MCM Hema tracer 804, LMS Co Ltd, Tokyo, Japan. b. Tokyo Kasei Industries Co, Tokyo, Japan

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in American Journal of Veterinary Research

PLRP Platelet- and leukocyte-rich plasma PMP Platelet membrane–derived microparticles PPP Platelet-poor plasma PRP Platelet-rich plasma PS Phosphatidylserine VWF:Ag von Willebrand factor antigen a. Cornell University Equine Park

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in American Journal of Veterinary Research