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essential medium–E a with 10% fetal bovine serum, b 5% serum replacement solution, c 2% penicillin-streptomycin solution, 1% amphotericin B solution, and 1% gentamicin sulfate solution. Harvested virus was quantified by use of a standard TCID 50 assay in

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in American Journal of Veterinary Research

procedures. The cells were maintained in cell line medium, which consisted of DMEM b with 1 g/L glucose, l -glutamine, and sodium pyruvate that contained 10% fetal bovine serum, c and penicillin (200 U/mL)-streptomycin d (200 μg/mL) at 37°C and 5% CO 2

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in American Journal of Veterinary Research

antimicrobials belonging to 12 drug classes: penicillin, oxacillin, ampicillin, and amoxicillin-clavulanic acid (β-lactams); gentamicin and neomycin (aminoglycosides); enrofloxacin, levofloxacin, ofloxacin, and moxifloxacin (fluoroquinolones); vancomycin (glyco

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in American Journal of Veterinary Research

: 73 – 77 . 15. Allen LJ , George LW , Willits NH . Effect of penicillin or penicillin and dexamethasone in cattle with infectious bovine keratoconjunctivitis . J Am Vet Med Assoc 1995 ; 206 : 1200 – 1203 . 16. Shryock TR , White

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in American Journal of Veterinary Research

. 7. Davis JL . Intravenous antibiotics for endophthalmitis . Am J Ophthalmol 1996 ; 122 : 724 – 726 . 10.1016/S0002-9394(14)70493-3 8. Rowley RA Rubin LF . Penetration of penicillin into the aqueous humor in the dog . Am J Vet Res 1969

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in American Journal of Veterinary Research

solution of Dulbecco modified Eagle medium and Ham F-12 nutrient mixture c supplemented with 5% fetal bovine serum, d 200 U of penicillin/mL, 200 μg of streptomycin/mL, e 0.5 μg of amphotericin B/mL, a 20 ng of epidermal growth factor/mL, f 5.6 μg of

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in American Journal of Veterinary Research

AC depth, CLT, and AxL values. Beginning 2 hours before the induction of anesthesia and continuing for a 48-hour period, penicillin G procaine m (22,000 U/kg, IV, q 6 h), gentamicin sulfate 11 (6.6 mg/kg, IV, q 24 h), flunixin meglumine o (1.1 mg

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in American Journal of Veterinary Research

composed of 50 µL of minimum essential medium-E with heat-inactivated 10% fetal bovine serum and 2% penicillin-streptomycin solution was added to plate wells along with 50 μL of test sample, and 2-fold serial dilutions were performed. The working dilution

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in American Journal of Veterinary Research

sera were heat-inactivated in a 56°C water bath for 30 minutes. Test medium consisting of 50 μL of Eagle minimum essential medium i with heat-inactivated 10% fetal bovine serum j and 2% penicillin-streptomycin solution was added to plate wells along

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in American Journal of Veterinary Research

fortified with 10% antimicrobial solution [streptomycin, penicillin, and amphotericin] l ), 2% concentration of low-melting-point 2-hydroxyethyl agarose powder, m and 1.7% concentration of sterile carbon suspension n was used to fill the corneas. The

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in American Journal of Veterinary Research