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15% horse serum, 15% yeast extract, 2 × 10 6 units of penicillin G, and 10% thallium acetate and incubated at 37°C in an atmosphere of 10% CO 2 in air. Another portion of sediment was inoculated onto an anaerobic blood agar plate (prereduced

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in American Journal of Veterinary Research

pituitary extract, h 1% penicillin-streptomycin, 1% l -glutamine, and 10% fetal calf serum. g Cell growth was assessed daily, and the culture medium was changed every 7 days. Endothelial cells were grown to confluence, and morphological characteristics

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in American Journal of Veterinary Research