Search Results

You are looking at 1 - 3 of 3 items for :

  • Cardiovascular System x
  • Refine by Access: All Content x
Clear All

Abstract

Objective—To compare mitochondrial complex I and complex IV activity in myocardial mitochondria of clinically normal dogs, clinically normal dogs exposed to inhalation anesthesia, and dogs affected with dilated cardiomyopathy.

Sample—Myocardial samples obtained from 21 euthanized dogs (6 clinically normal [control] dogs, 5 clinically normal dogs subjected to inhalation anesthesia with isoflurane prior to euthanasia, 5 dogs with juvenile-onset dilated cardiomyopathy, and 5 dogs with adult-onset dilated cardiomyopathy).

Procedures—Activity of mitochondrial complex I and complex IV was assayed spectrophotometrically in isolated mitochondria from left ventricular tissue obtained from the 4 groups of dogs.

Results—Activity of complex I and complex IV was significantly decreased in anesthetized dogs, compared with activities in the control dogs and dogs with juvenile-onset or adult-onset dilated cardiomyopathy.

Conclusions and Clinical Relevance—Inhalation anesthesia disrupted the electron transport chain in the dogs, which potentially led to an outburst of reactive oxygen species that caused mitochondrial dysfunction. Inhalation anesthesia depressed mitochondrial function in dogs, similar to results reported in other species. This effect is important to consider when anesthetizing animals with myocardial disease and suggested that antioxidant treatments may be beneficial in some animals. Additionally, this effect should be considered when designing studies in which mitochondrial enzyme activity will be measured. Additional studies that include a larger number of animals are warranted.

Full access
in American Journal of Veterinary Research

subsequent impairment of cardiac myocyte function and cardiac remodeling. 6,44–46 Most dogs with systemic diseases of the present study were oncology patients and euthanized because of malignant, final-stage tumors, and an activation of the inflammatory

Full access
in American Journal of Veterinary Research

propagation velocity is a preload insensitive index of left ventricular relaxation: animal and human validation . J Am Coll Cardiol 2000 ; 35 : 201 – 208 . 10.1016/S0735-1097(99)00503-3 44. Theilen GH . Chapter 6: veterinary medical oncology . In

Full access
in American Journal of Veterinary Research