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medium (6) via aerosolization on day 0. All calves were administered aerosolized ovalbumin once daily on days 1 through 6 and day 15. Calves were monitored daily for clinical signs of illness throughout the 16-day study period. A nasal swab was collected

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in American Journal of Veterinary Research

for bacterial culture have been investigated and include nasal swabs, transtracheal swabs, transtracheal washes, NPSs, BALs, lung lavages, lung swabs, and lung tissue specimens. 3–9 Each method has strengths and limitations and varies in terms of the

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in American Journal of Veterinary Research

inclusion in the study were negative results of M bovis culture of a nasal swab sample, negative results of a PCR assay for M bovis , low M bovis ELISA serum titer (the highest titer for a calf included in the study had an ELISA value that was 29.6% of

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in American Journal of Veterinary Research