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horses with neurologic signs. Blood, nasal swab specimen, and CSF sampling and processing —Blood samples and nasal swab specimens were obtained on day −1 and 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, and 21 days after infection. Blood was drawn into 10-mL

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in American Journal of Veterinary Research

hours after birth from a small subset (n = 34) of calves for which both birth and the first nursing episode were observed. A blood sample (approx 9 mL at initial vaccination and 25 mL at preconditioning and weaning) for serum acquisition and nasal swab

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in American Journal of Veterinary Research

strains of IBR and PI3. For each calf, body weight was measured on days −21 (arrival at research facility), 7, 14, 21, and 28. A nasal swab specimen was obtained from each nostril immediately prior to administration of the vaccine or saline solution

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in American Journal of Veterinary Research

nebulizer via a bleeder-type cutoff valve attachment j and was positioned on a rolling cart adjacent to a hydraulic chute. Individual steers were restrained securely in the hydraulic chute, and nasal swab specimens were collected to determine prior exposure

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in American Journal of Veterinary Research

inoculation, pigs were intranasally inoculated with PRRSV MN-30100 or MN-184 according to a described method. 11 Control pigs were anesthetized and sham-inoculated with sterile saline solution via the same intratracheal procedure. Nasal swab specimens were

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in American Journal of Veterinary Research

studies. 15,24 Nasal swab specimens were collected from only the control calves and calves exposed to the HoBi-like virus; these specimens were collected on days −2, 3, 6, 9, 11, and 13. Results A decrease in the number of circulating WBCs was

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in American Journal of Veterinary Research

performed on ear notch samples, PCR assay performed on serum samples, and VI performed on serum and nasal swab specimens in a 96-well infectivity assay 14 were selected for use in the study. All PI cattle were confirmed positive for BVDV1b by sequencing of

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in American Journal of Veterinary Research

. − = Negative result. + = Positive result. B = Buffy coat. C = Calf. F = Fawn. n = Not done. N = Nasal swab specimen. O = Oral swab specimen. R = Rectal swab specimen. Following cohabitation, calves and fawns were commonly seen sharing the same pen area

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in American Journal of Veterinary Research

Bacterial isolates cultured from antemortem oropharyngeal or nasal swab specimens obtained from free-ranging bighorn sheep submitted to the Caine Veterinary Teaching Center were included in the present study. However, isolates from a 1995–1996 respiratory

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in American Journal of Veterinary Research

respiratory infection: nasal swab versus nasopharyngeal aspirate using realtime polymerase chain reaction . Eur J Clin Microbiol Infect Dis 2010 ; 29 : 365 – 371 . 10.1007/s10096-009-0865-7 22. Papadopoulos NG Stanciu LA Papi A , et al. A

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in American Journal of Veterinary Research