Search Results

You are looking at 1 - 10 of 28 items for :

  • Clinical Pathology x
  • Refine by Access: All Content x
Clear All

Vitamin D–binding protein is part of the extracellular actin scavenging system that facilitates clearance of actin from the circulation. 1 Actin is an intracellular structural protein that is released into the circulation after cell injury and

Full access
in American Journal of Veterinary Research

, 13 – 16 Serum H3 and serum H4 are implicated in the pathogenesis of sepsis-induced tissue injury, and neutralization of these factors by specific anti-histone antibodies or nonanticoagulant heparin significantly decreases their deleterious effects. 9

Full access
in American Journal of Veterinary Research

Abstract

Objective

To test the hypothesis that the derangement of myocardial energy homeostasis characteristic of idiopathic dilated cardiomyopathy (IDCM) of Doberman Pinschers was attributable to deficiency of creatine kinase (CK) isoenzyme MB relative to CK isoenzyme MM and troponin-T (Tn-T).

Animals

9 Doberman Pinschers with advanced congestive heart failure attributable to IDCM and 9 mixed-breed dogs without cardiac disease (controls).

Procedure

Myocardial myofibrillar CK-MM, mitochondrial CK, and cytosolic CK-MB activities were determined in comparison with the concentration of cardiac-specific, myofibrillar protein Tn-T. Myocardial biopsy specimens were obtained after euthanasia and were ultrafrozen. Isoenzymes of CK were separated eloctrophoretically, and their activity was determined, using a coupled-enzyme, kinetic, fluorescent assay and densitometry. Troponin-T content was determined by immunoassay.

Results

Myofibrillar CK-MM and mitochondrial CK activities and Tn-T content were 25% lower in the dogs with IDCM than in controls, whereas cytosolic CK-MB activity was 50% lower.

Conclusion

Deficiency of CK-MB activity is a component of and may have a central role in the energy deficiency characteristic of failing myocardium in IDCM of Doberman Pinschers. A side-product of this study was the observation that, in dogs, CK-MB activity and Tn-T content of myocardium are sufficiently high to be candidate serum markers for active cardiac injury. (Am J Vet Res 1997;58:11–16)

Free access
in American Journal of Veterinary Research

SUMMARY

Pneumonic pasteurellosis was experimentally induced in calves by inoculation of 5 × 108 Pasteurella haemolytica organisms into the right diaphragmatic lung lobe. Blood and bronchoalveolar lavage fluid samples were obtained prior to inoculation and at postinoculation hour (pih) 2, 4, and 6. Calves developed acute lung injury, characteristic of pneumonic pasteurellosis. Lesions were found only in the right diaphragmatic lobe. By pih 4, significant (P < 0.01) increases were detected in lavage fluid total cell count, neutrophil count, total protein and albumin concentrations, and alkaline phosphatase (alp) and lactic dehydrogenase (ld) activities. Myeloperoxidase and elastase activities did not increase. Neutrophil depletion ameliorated the lung lesions and prevented the increase in lavage fluid cell count, total protein, and albumin concentrations and alp and ld activities. Treatment with the iron chelator, deferoxamine mesylate-hydroxyethyl starch, attenuated the increase in total protein and albumin concentrations and alp and ld activities at pid 4, but not pih 6. Treatment with a neutrophil function inhibitor, pentoxifylline, prevented the increase in lavage fluid neutrophil numbers, but accentuated the increase in total protein and albumin concentrations, and alp, ld, myeloperoxidase, and elastase activities.

Free access
in American Journal of Veterinary Research

Measurement of concentrations of cTnI, a myofibril protein that regulates contraction of myocardium, is considered the gold standard diagnostic test for detection of cardiac injury in humans. 1,2 There is > 96% homology between cTnI of humans and

Full access
in American Journal of Veterinary Research

. Therefore, we believe that depletion of peripheral blood neutrophils in cats may be primarily attributable to segregation of neutrophils in some area or areas of the body. Use of FTY720 can reduce ischemia-reperfusion injury in rats via inhibition of T

Full access
in American Journal of Veterinary Research

venous blood samples (systemic circulation) of horses with clinical colonic ischemia that have extensive and severe colonic injury. 1,3 Such changes were not detected in the jugular venous blood samples obtained from horses of the present study, possibly

Full access
in American Journal of Veterinary Research

eosinophils has been described for horses with experimentally induced acute colitis 20 and in horses with experimentally induced ischemia and reperfusion injury. 21 Eosinophil accumulation in the gastrointestinal mucosa can also be associated with parasitism

Full access
in American Journal of Veterinary Research

injury. 2–5 Inflammatory cytokines such as interleukin-1β, interleukin-6, and tumor necrosis factor-α are responsible for upregulation of SAA synthesis. 1 Studies of the SAA response to joint disease in horses are limited, but it is known that SAA

Full access
in American Journal of Veterinary Research

promotes thromboembolic events and potentiates inflammatory reactions that can lead to sepsis, septic shock, and MODS. 9–13 Once platelets are activated and tethered to the site of endothelial injury, they recruit and activate neutrophils through the

Full access
in American Journal of Veterinary Research