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were accessed via celiotomy, perfused in situ with 4 volumes of 25 mL of saline (0.9% NaCl) solution, excised, and placed on ice. Liver tissue (9 to 11 g of wet wt), devoid of connective and vascular tissue, was homogenized at 4°C with 5 volumes of 0

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in American Journal of Veterinary Research