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beads used in the study were donated by Wedgewood Pharmacy. ABBREVIATIONS CSH Calcium sulfate semihydrate IC 50 Molar concentration of compound that inhibits specific activity by 50% Footnotes a. Hess T, Miller J, Fettig A, et al

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in American Journal of Veterinary Research

resected tumors. 1–10 However, many of these delivery systems are not commercially available, have resulted in unacceptable regional complications, or have resulted in minimal improvement in clinical outcome. 5,8,10,11 Carboplatin-impregnated calcium

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in American Journal of Veterinary Research

without causing tissue reactions. 17–19 Gelatin sponges impregnated with chemotherapeutics have been used in chemoembolization. 15,20,21 Calcium sulfate is slowly absorbed from tissues, with radiographic disappearance of the beads by 5 weeks after

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in American Journal of Veterinary Research

-depleted plasma (canine, factor-replete human, or specific factor-deficient human samples) was added, followed by 50 μL (15mM) of calcium chloride-containing fluorogenic thrombin substrate. x Fluorescence was then measured every 60 seconds at 360/460 nm for 2

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in American Journal of Veterinary Research

twice with HEPES buffer (10mM HEPES, 137mM sodium chloride, 5mM calcium chloride, 4mM potassium chloride, 10mM glucose, and 0.5% BSA; pH, 7.4). Activated recombinant human activated factor VII l (1nM) diluted in HEPES buffer was added for 10 minutes at

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in American Journal of Veterinary Research

. In humans, miR-497-5p is associated with malignancy in angiosarcoma and has been shown to directly target KCa3.1, a calcium-activated potassium channel. 38 Such potassium channels have been previously shown to regulate activity of cancer cells

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in American Journal of Veterinary Research

; black box), and calcium-lipid—binding region (C2; dark-gray box) are illustrated. PDZ = PSD-95/Dlg/ZO-1 homology domain-binding motif. In panel B, the number above each column is the number of amino acid residues from the N-terminus or nucleotides from

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in American Journal of Veterinary Research

approximately 70% confluence in a 10-cm 2 culture dish and then cotransfected with lentiviral shuttle vector (10 μg) and packaging vectors pFIV-34N (10 μg) and pVSVG (vesicular stomatitis virus, protein G ; 10 μg) by use of a calcium phosphate reagent. Sixteen

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in American Journal of Veterinary Research

Tumor invasion, metastasis, and angiogenesis require controlled degradation of the ECM. There are several families of ECM-degrading enzymes, the most extensive of which are the MMPs. They belong to a multigene family of zinc-containing, calcium

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in American Journal of Veterinary Research