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fed milk substitute . J Hyg (Lond) 1983 ; 91 : 33 – 45 . 10.1017/S0022172400060009 13 Bauer AW Kirby WM Sherris JC , Antimicrobial susceptibility testing by a standardized single disk method . Am J Clin Pathol 1966 ; 45 : 493 – 496 . 10

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in American Journal of Veterinary Research

equine specimens that were submitted to the Cornell University AHDC between January 1, 2001, and December 31, 2013, and subsequently underwent antimicrobial susceptibility testing. It was assumed that specimen submission by practitioners was either

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in American Journal of Veterinary Research

Veterinary and Microbiological Sciences at North Dakota State University during January to May 2010. Antimicrobial susceptibility testing —Susceptibility profiles of the E coli isolates were determined for 17 antimicrobial agents via the broth dilution

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in American Journal of Veterinary Research

equine origin; these were not investigated further. Each remaining isolate was suspended in cation-adjusted Mueller-Hinton broth (1 × 10 5 CFUs/mL) and transferred to a commercially available antimicrobial susceptibility test plate that was read after 24

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in American Journal of Veterinary Research

/cm, with a cooling temperature of 14°C. Gels were stained with ethidium bromide (1 μL/400 mL of water) for 30 minutes, destained with water for 75 minutes, and photographed. Antimicrobial susceptibility testing — Escherichia coli isolates were tested for

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in American Journal of Veterinary Research

However, to the author's knowledge, the susceptibility of MAP to gallium has not been established. Protocols for antimicrobial susceptibility testing of MAP have not yet been standardized, but typically, variations of the broth macrodilution method used

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in American Journal of Veterinary Research

CFUs (as determined by enumeration on Müeller-Hinton agar b ). This dose was consistent with doses used in another study. 17 Salmonella isolation, antimicrobial susceptibility testing, and quantification —Fecal samples were collected from each pig on

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in American Journal of Veterinary Research

this study. Further analysis was performed on the 357 isolates that fit these criteria. Antimicrobial susceptibility testing —Antimicrobial MICs for S aureus isolates were determined by use of a semiautomated antimicrobial susceptibility system

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in American Journal of Veterinary Research