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obtained from control calves. Another CMI assay, flow cytometry, has been used to evaluate specific proliferation of MAP-activated lymphocyte subsets. 14 In addition, an ELISA for IFN-γ content in the culture supernatant was evaluated in a concurrent

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in American Journal of Veterinary Research

binds to pathogens. Once IgM is bound to a pathogen, the pentameric IgM-antigen complexes activate complement and increase phagocytosis. Equine IgG1 binds to Fc receptors, induces respiratory burst from leukocytes, and activates the classical complement

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in American Journal of Veterinary Research

as an adjuvant to allow the virus to persist at the target site and increase the chances of activating an immune response. Future formulations should include bile inhibitors, mineral oil, or both. The alginate-encapsulated vaccine failed to

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in American Journal of Veterinary Research

RL Alt DP Palmer MV , et al. A Leptospira borgpetersenii serovar Hardjo vaccine induces a Th1 response, activates NK cells, and reduces renal colonization . Clin Vaccine Immunol 2011 ; 18 : 684 – 691 . 10.1128/CVI.00288-10 18. Bolin

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in American Journal of Veterinary Research

large (ie, activated) subpopulation of PBMCs in response to each stimulant was also measured via flow cytometry. For cells dual stained for CD25 and CD4, CD8, or the γΔ T-cell receptors, the percentage of double-positive cells multiplied by the mean

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in American Journal of Veterinary Research

3B served as a positive control. Following a 10-minute activation at 95°C, reaction preparations were cycled in a ther mocycler h for 40 cycles consisting of 30 sec onds at 95°C, 30 seconds at 44°C, and 60 seconds at 72°C. Products were analyzed by

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in American Journal of Veterinary Research

the mystery swine disease in the growing pig, in Proceedings . Mystery Swine Dis Comm Meet 1990 ; 41 – 49 . 5 Molitor TW Xiao J Choi CS . PRRS virus infection of macrophages: regulation by maturation and activation state, in

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in American Journal of Veterinary Research
Full access
in American Journal of Veterinary Research