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performed on recalcified citrated whole blood with an activator such as tissue factor or kaolin added 30 to 90 minutes after blood collection to assist coagulation, making this technique practical for use in veterinary clinical settings. In dogs, reference

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in American Journal of Veterinary Research

crude indicators of concentrations of soluble coagulation factors, fibrin formation, and platelet function, but the interpretation of these tests is subjective and thus imprecise. 3 Widely used plasma-based tests (eg, activated partial thromboplastin

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in American Journal of Veterinary Research

–3 Platelet activation leads to expression of adhesion molecules on their surface, mediating platelet aggregation and interaction with other cells such as endothelial cells and leukocytes. 4–6 Once activated, platelets release and express P-selectin (CD62P

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in American Journal of Veterinary Research

generation. In the past, platelets were considered a homogenous population with equal potential for activation, which would lead to stimulation of local generation of thrombin. However, recent evidence suggests that all activated platelets are not equal and

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in American Journal of Veterinary Research

blood product, any information gathered within 48 hours after the transfusion was excluded. Coagulation monitoring included dual-channel kaolin-activated thromboelastography and determination of BMBT, PT, aPTT, D-dimer concentration, and fibrinogen

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in American Journal of Veterinary Research

convincing evidence that circulating leukocytes are activated 5 and that the number of leukocytes located in the skin and laminar tissue of the hoof increases significantly in horses that develop clinical signs of Obel grade 1 laminitis. 6–8 These findings

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in American Journal of Veterinary Research

coagulation in vitro and result in shortening of activated clotting time, 1 platelet activation or clumping, and clotted samples. 2 Other factors include prolonged venous stasis, inappropriate filling and mixing of blood tubes, 3 and variation within and

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in American Journal of Veterinary Research

Endothelial cell activation and dysfunction are key events in sepsis, leading to leukocyte adhesion, altered vasomotor tone, and increased vascular permeability. 1 The endothelial-specific angiopoietin-Tie ligand receptor system is an important

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in American Journal of Veterinary Research

cycle time. The SrT was the amount of time required for a paw to complete a gait cycle, and SrL was defined as the distance between 2 successive strikes of the same paw. An NS was the number of sensors activated by each paw. The TPI was defined as the

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in American Journal of Veterinary Research

. The percentage of viable cells also differed between CLM and LDH histochemistry. This difference may be related to the enzymes responsible for the activation of the stain and their susceptibility to thermal injury. Results of TUNEL indicate that

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in American Journal of Veterinary Research