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by triggering the production and release of endogenous mediators of inflammation, including the secretion of cytokines such as TNF-α and IL-6. 13–15 Nearly all LPS effects are strictly dose related. 16 Study of the physiologic effects of LPS

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in American Journal of Veterinary Research

Abstract

Objective

To develop and define a model of acute synovitis/capsulitis in the equine metacarpophalangeal joint (fetlock) to study clinical effects of diagnostic or therapeutic procedures.

Animals

5 adult Standardbreds.

Procedure

Polyvinyl alcohol foam particles were injected into the left front fetlock of horses; the right front fetlock was used as a control. Horses were examined clinically and for lameness on a regular basis. Blood samples were taken to measure routine variables. Synovial fluid samples were collected from both fetlocks, and macroscopic, microscopic, and biochemical variables were measured. The effects of local diagnostic nerve blocks and intra-articular anesthesia of the fetlock on ensuing lameness were determined on days 2 and 3. After macroscopic evaluation at necropsy, histologic examination was performed on the fetlock cartilage and capsule.

Results

Changes in synovial fluid variables included increased TNF-α and matrix metalloproteinases values and in other indicators of acute inflammation. With respect to application of local nerve blocks, lameness decreased most after a combination of metacarpal and palmar nerve blocks, whereas lameness could only partially be blocked by intra-articular anesthesia. At necropsy, mild chronic inflammation of the synovium and deeper layers of the joint capsule was found; polyvinyl alcohol particles had been incorporated into the joint capsule. There was no evidence of articular cartilage damage.

Conclusions

Injection of polyvinyl alcohol foam particles induces transient synovitis/capsulitis in the fetlock, mimicking clinical joint disorders. The model does not seriously affect the horse's long-term well-being. Such model serves well for studying the effect of diagnostic or therapeutic procedures. (Am J Vet Res 1998;59:978–985)

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in American Journal of Veterinary Research

traps). 9 – 12 Extracellular his-tones bind to toll-like receptors, leading to increased production of proinflammatory cytokines (eg, TNF-α and IL-6). 11 – 13 Moreover, experimental administration of histones to mice and monkeys induces neutrophil

Full access
in American Journal of Veterinary Research