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were seeded in 25-cm 2 flasks and grown to confluence. The culture supernatant was removed and replaced with fresh DMEM. The cells were then incubated with recombinant human TNF-α n (10 ng/mL of DMEM) for 24 hours. All cell culture supernatants were

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in American Journal of Veterinary Research

product was measured at a wavelength of 550 nm by use of an ELISA plate reader. i Assessment of procoagulant activity and TNF-α production induced by equine mononuclear cells exposed to BWE —Five hundred microliters of equine mononuclear cells

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in American Journal of Veterinary Research

-Lamoureux A , Maghni K , Lavoie J-P . Optimization of a procedure to accurately detect equine TNFα in serum samples . Vet Immunol Immunopathol 2010 ; 138 : 118 – 123 . 10.1016/j.vetimm.2010.06.018 19. Mire-Sluis AR , Gaines-Das R , Thorpe

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in American Journal of Veterinary Research