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more recent study 8 revealed that clinical mastitis in nonvaccinated cows was nearly 3 times as likely to result in culling or death as clinical mastitis in J5-vaccinated cows. Additionally, higher serum anti-J5 E coli IgG concentration was

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in American Journal of Veterinary Research

horses by means of WNV-specific IgM detection. Recent reviews 16,17 of WNV diagnosis and prevention techniques conclude that currently available WNV vaccines decrease clinical disease in horses and the modified-live virus vaccine induces an IgG response

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in American Journal of Veterinary Research

with high animal density 37,42,43 ; cattle housed in barns with tie stalls or stanchions; and the same equipment used to handle manure and feed 37 ) and immunologic risks (ie, low serum IgG titers against BCoV in calves at arrival to feedlots 36,44 or

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in American Journal of Veterinary Research

superior to a parenteral route of vaccination, which stimulates a high systemic IgG response. Bovine ocular IgG arises primarily from plasma, 32 and results of another study 30 indicate that an increase in the systemic IgG response against MbxA results in

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in American Journal of Veterinary Research

vaccine will not cause clinical signs of strangles in vaccinated ponies, will result in increased concentrations of serum anti- S equi IgG, will not be recovered at > 3 days after vaccination or administration of a booster vaccination, and will not result

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in American Journal of Veterinary Research

in aliquots at −20°C until analyzed for serum total IgG concentration and SNA titers against BHV1, BVDV1, and BRSV The nasal secretion samples were mixed with an equal volume of 0.1% Tween in PBSS, vortexed briefly, and then centrifuged at 834 × g

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in American Journal of Veterinary Research

-cell subsets and IFN-γ assay. Primary monoclonal antibodies Isotype or label Secondary antibody or reagent conjugates Mouse anti-bovine CD4 f IgG1 Goat anti-mouse IgG1, Phycoerythrin-Texas red g Mouse anti-bovine CD8 h

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in American Journal of Veterinary Research

as an indicator of fecal inhibition. 18 Serum was harvested from blood samples to determine the concentration of total solids by use of a refractometer as well as to measure the amount of IgG antibody against L intracellularis via IPMA, as

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in American Journal of Veterinary Research
Full access
in American Journal of Veterinary Research

wells in a separate plate. At the end of the incubation period, each well of every plate was washed 3 times with PBST (300 μL/well). The PBST was decanted, and 100 μL of goat anti-bovine IgG l diluted 1:5,000 in 1% casein was added to each well. The

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in American Journal of Veterinary Research