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proteinuria. Additionally, in a recent study 20 of dogs, there was a high correlation between SDS-AGE qualitative findings and quantitative measurements of urine albumin and IgG. 20 Because protein bands in gels may be used for identification purposes 26

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in American Journal of Veterinary Research

antibody as used in western blotting procedures. Antigen retrieval was performed at 99°C for 15 minutes in 0.1M EDTA-NaOH buffer (pH, 8.8). Anti-rabbit IgG coupled to fluorescent dye t was used for visualization of ligated fibronectin antibody. A

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in American Journal of Veterinary Research

.9%, respectively. Quantitation of UAlb —The concentration of UAlb was determined by use of an ELISA with a doubleantibody sandwich technique. Wells of microtiter plates were coated with an IgG fraction of affinity-purified goat anti-dog albumin l (1 mg/mL in 50

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in American Journal of Veterinary Research

.00268M potassium chloride). Standards were prepared at 0, 0.003, 0.01, 0.03, 0.1, and 0.3 μg of Gd-DPTA·mL −1 , and serum samples were diluted at 1:400. Aliquots (50 μL) of standard or diluted sample were added to goat anti-rabbit IgG–coated microtiter

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in American Journal of Veterinary Research