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SUMMARY

Toxoplasma gondii-specific IgA, IgM, and IgG were measured by ELISA in the serum and aqueous humor of 29 client-owned cats with endogenous uveitis and 7 specific-pathogen-free cats tested sequentially for 20 weeks after inoculation with T gondii. Local antibody production in aqueous humor was estimated by multiplying the aqueous humor-to-serum T gondii-specific antibody ratio by the serum-to- aqueous humor total IgG (C value) or calicivirus-spe- cific IgG (CTC value) ratio. Evidence for local production of antibody in aqueous humor was defined as C value greater than 8 or CTC value greater than 1. Toxoplasma gondii-specific IgM CTC values, IgG CTC values, or IgA CTC values greater than 1 were detected in the aqueous humor of 18 of 29 (62.1%) client- owned cats with endogenous uveitis; 2 cats had IgA CTC values greater than 1 without detectable IgM or IgG in aqueous humor. Toxoplasma gondii-specific IgM was not detected in the aqueous humor of experimentally inoculated cats before or after inoculation. Immunoglobulin G C values greater than 8 were detected in all 7 experimentally inoculated cats and ranged from 10.4 to 145.5.

Immunoglobulin G C values greater than 8 were first detected 4 to 8 weeks after T gondii inoculation and were undetectable by week 16 after inoculation. Immunoglobulin A C values greater than 8 were detected in 4 of 7 cats and ranged from 12.7 to 264.3. Immunoglobulin A C values greater than 8 were first detected 4 to 8 weeks after inoculation, and were detected in 2 cats during week 20 after inoculation. It was concluded that some cats infected with T gondii develop detectable concentrations of T gondii-specific IgA in aqueous humor.

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in American Journal of Veterinary Research

, Mass. h. Thermo Scientific, Waltham, Mass. i. Bio-Rad Laboratories Inc, Hercules, Calif. j. p-VASP (16C2), Santa Cruz Biotechnology, Dallas, Tex. k. Goat anti-mouse IgG-HRP, Santa Cruz Biotechnology, Dallas, Tex. l. SuperSignal West Femto

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in American Journal of Veterinary Research

and phycoerythrin-conjugated CD8 +p for 15 minutes at 4°C in the dark. Concentration of the antibodies was 1 μg of IgG/10 μL of PBS solution. Cells were washed twice with 200 μL of flow cytometry buffer (97% PBS solution and 3% heat-inactivated fetal

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in American Journal of Veterinary Research

. Covalent binding of polymyxin B to human IgG results in nontoxic, dose-dependent protection against death in a murine sepsis model. 43 Polymyxin B nonapeptide, a cleavage product of polymyxin B, retains endotoxin-binding activity but is much less potent

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in American Journal of Veterinary Research