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sporocysts to horses elicited synthesis of S neurona –specific IgG and IgM in blood and CSF, but protozoa were not detected in the CNS tissues of challenged horses. Neurologic signs and histologic evidence of CNS inflammation have developed inconsistently in

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in American Journal of Veterinary Research

antigen EIA (AgEIA) and MiraVista canine Coccidioides IgG EIA (AbEIA). Samples were submitted in batches of approximately 15 to 20 to MiraVista Diagnostics for the panel of tests. The frozen samples were shipped overnight in 3 batches approximately 4

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in American Journal of Veterinary Research

-mouse IgG 2a (1:25) h was used to label anti-MHC I and anti-MHC II antibodies by incubation for 30 minutes on ice. Aliquots containing an equal number of cells were incubated with respective isotype control antibodies under the same conditions. Cells

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in American Journal of Veterinary Research

GFAP astrocytopathy, has recently been described in humans and is characterized by the expression of an astrocytic autoantibody called GFAP autoantibody. 12 , 13 , 14 More specifically, IgG binding to the GFAP-α is more accurate for diagnosis of GFAP

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in American Journal of Veterinary Research

microwave processor. Then, the sections were incubated with a primary antibody in appropriate treatment for each antibody. Sections were sequentially incubated with biotinylated goat anti-rabbit IgG a (diluted 1:400) or biotinylated goat anti-mouse IgG a

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in American Journal of Veterinary Research

(clone No. 2F11) t to identify neuron markers. After being washed with PBS solution, the cells were incubated and exposed to goat anti-mouse IgG–conjugated secondary antibody u for 1 hour in darkness at room temperature. Cells were also incubated

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in American Journal of Veterinary Research

-brainbarrier damage (albumin quotient in the reference range) and a normal to high ratio of IgG in serum and CSF (IgG index) are considered indicative of intrathecal production of antibodies in response to the parasite in the CNS. 1,7 Natural transfer of antibodies

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in American Journal of Veterinary Research

anti-mouse IgG antibody. d Protein bands were evaluated by use of chemiluminescence. d Rhodamine 123 efflux studies —The OS2.4/Doxo cells (2 × 10 6 ) were grown in monolayer to approximately 80% confluency (overnight incubation) in 25-mm 2 flasks

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in American Journal of Veterinary Research

2011 ; 13 : 687 – 693 . 10.1016/j.jfms.2011.05.014 37. Klang A Schmidt P Kneissl S , et al. IgG and complement deposition and neuronal loss in cats and humans with epilepsy and voltage-gated potassium channel complex antibodies . J

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in American Journal of Veterinary Research

through the cross-linkage of IgG and IgM to microbial or tissue antigens. 61 Therefore, this finding would not allow distinction between an autoimmune or microbial etiology for Greyhound meningoencephalitis. 62 Interestingly, however, biosynthesis of

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in American Journal of Veterinary Research