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. Primary antibody Dilution Secondary antibody Mouse monoclonal (BC-3) to human aggrecan ARGSVIL s 1:2,000 Sheep polyclonal to mouse IgG H&L (HRP) t Rabbit polyclonal to versican (V0 and V1) DPEAAE (within β domain) u 1

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in American Journal of Veterinary Research

were prepared with commercially available normal rabbit IgG or normal goat IgG isotype control primary antibodies. d Control slides were imaged to determine the amount of background staining. The exposure time used to accurately image the control slide

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in American Journal of Veterinary Research

was washed 3 times in TBS-T solution. The membrane was then incubated with donkey anti-goat IgG polyclonal antibodies linked to horseradish peroxidase g diluted (1:2,000) in TBS-T solution with 5% nonfat milk for 1 hour at room temperature. The

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in American Journal of Veterinary Research

:100) for virally treated cells. After several washes in PBS solution, coverslips were incubated for 1 hour with secondary antibody (goat antimouse dd or goat anti-rabbit IgG, ee as appropriate [diluted 1:500]), washed in PBS solution, and mounted in

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in American Journal of Veterinary Research

humoral immune responses in chickens fed lactic acid bacteria indicated a strain-dependent response, with expression of relevant IgG and IgM titers in chickens fed only 1 of the 5 probiotic strains tested. 56 Immunomodulation by probiotics and synbiotics

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in American Journal of Veterinary Research