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mucosal damage, thereby preventing or minimizing clinical disease. Because administration of adsorbents to foals coincides with the period of passive transfer of colostral antibodies, 31–33 the effect of adsorbents on immunoglobulin (IgG) absorption must

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in American Journal of Veterinary Research

gondii inoculation of each cat had negative results when tested for T gondii oocysts and other enteric parasite eggs, cysts, or oocysts. Anti– T gondii IgM and IgG ELISA Serum samples for ELISAs were obtained from each cat at the same times as

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in American Journal of Veterinary Research

for basement membranes and porcine fibrinogen or fibrin by use of a monoclonal antibody against porcine fibrin or fibrinogen a followed by biotinylated equine anti-mouse IgG, which were then developed by use of avidin-conjugated horseradish peroxidase

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in American Journal of Veterinary Research

once with distilled water. An affinity-purified, fluorescein isothiocyanate–conjugated, rabbit anti-canine IgG (H + L) antibody m (dilution, 1:100) was added to each well; wells were incubated for 30 minutes and then washed. Slides were coated with 10

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in American Journal of Veterinary Research

include IgG, complement, 11,23 and other plasma proteins), modest neutrophilic glomerular cellular infiltrates, and, ultimately, glomerular sclerosis. 6,10,11,13,24,25 Proteinuria and edema (nephrotic syndrome) are often characteristic of the clinical

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in American Journal of Veterinary Research

.05% Tween 20 i and probed with peroxidase-labeled goat anti-mouse IgG l for 1 hour on a shaker at room temperature. Five additional washes were performed, and the protein was detected by enhanced chemiluminescence. m A biotinylated protein ladder n was

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in American Journal of Veterinary Research

IgG fraction against mouse IgG h (diluted 1:2,000 in 5% nonfat dry milk) was added to the membranes, which were then incubated for 1 hour at 37°C. Membranes underwent 3 washes (10 min/wash) with fresh TBSS at 37°C, 1 wash with citrate-EDTA buffer (10

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in American Journal of Veterinary Research

min/wash) with fresh TBSS at 37°C. Horseradish peroxidase–conjugated goat IgG fraction against mouse IgG h (diluted 1:2,000 in 5% nonfat dry milk) was added to the membranes, which were then incubated for 1 hour at 37°C. Membranes underwent 3 washes

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in American Journal of Veterinary Research

were incubated at 22° to 25°C for 1 hour with continual mixing by an orbital shaker. The nitrocellulose membranes were washed 5 times. Then, a 1:1,000 dilution of peroxidase-labeled, secondary antibodies (goat–anti-mouse IgG q [glyceraldehyde 3

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in American Journal of Veterinary Research

-labeled antibody against cat IgG, k followed by addition of substrate. l A strong positive result was recorded if the protein band was easily visible in the nitrocellulose strip. A weak positive result was recorded if the band was faint and negative if no band

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in American Journal of Veterinary Research