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) antibody. f Negative controls for each tissue specimen were treated in a similar manner except that the primary antibody was not applied. Biotinylated horse anti-goat IgG (secondary) antibody was applied at a 1:10 dilution; slides were then incubated with

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in American Journal of Veterinary Research

with a goat anti-rabbit IgG horseradish peroxidase–conjugated secondary antibody f (1:5,000 diluted in 5% nonfat milk for 1.5 hours at room temperature) and a standard film developer. Band intensities were measured via densitometry. Total and HMW

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in American Journal of Veterinary Research

-polyhistidine, Santa Cruz Biotechnology, Santa Cruz, Calif. l. Normal rabbit serum, Bethyl Laboratories Inc, Montgomery, Tex. m. Fluorescein isothiocyanateconjugated polyclonal goat antirabbit IgG, Jackson ImmunoResearch, West Grove, Pa. n. ProLong Gold with DAPI

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in American Journal of Veterinary Research

, Tokyo, Japan. j. Op'-DDD, Yakult Honsha Co, Tokyo, Japan. k. Pentobarbital, Dainippon Pharmaceutical Co, Tokyo, Japan. l. Monoclonal mouse anti-adrenocorticotropin, Dako Japan Co, Kyoto, Japan. m. Pit-1 rabbit polyclonal IgG, Santa Cruz

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in American Journal of Veterinary Research

-Mouse IgG:HRP, Serotec, Oxford, England. l. SAS, version 9.1, SAS Institute Inc, Cary, NC. References 1. Scott-Moncrieff JC . Atypical and subclinical hyperadrenocorticism . In: Bonagura JD Twedt DC , eds. Kirk's current veterinary therapy

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in American Journal of Veterinary Research

1 hour to block nonspecific binding sites. After blocking, slides were washed with PBS solution, guinea pig anti–porcine insulin primary IgG antibody g was applied (1:50 dilution in PBS solution for 32 minutes), and 3,3′-diaminobenzi-dine was used

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in American Journal of Veterinary Research

c Volume of distribution in central compartment Vd ss Volume of distribution at steady state a. SNAP Foal IgG Test, IDEXX Laboratories Inc, Westbrook, Me. b. Immulite, Diagnostics Product Corp, Los Angeles, Calif. c. Dexamethasone

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in American Journal of Veterinary Research

were created with PBS solution–Tween-20 containing 2% BSA). Plates were incubated for 2 hours at room temperature (22°C), and the wells then were washed. Goat-origin anti-canine IgG conjugated to horseradish peroxidase m (100 μL, diluted 1:5,000 in PBS

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in American Journal of Veterinary Research

), mouse IgG was used instead of the primary antibody. Sections were stained with chromagen 3.3′-diaminobenzidine tetrahydrochloride m twice for 5 minutes (with an intervening Tris-buffered saline with Tween wash) followed by counter-staining with Mayer

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in American Journal of Veterinary Research

porcine GH standards (1 to 100 ng/mL), antiserum produced in guinea pigs inoculated with porcine GH, iodine 125 ( 125 I)–labeled porcine GH, and a precipitating reagent complex (goat anti–guinea pig IgG serum in 3% polyethylene glycol and 0.05% nonionic

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in American Journal of Veterinary Research