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Sections were also incubated with mouse anti-human αSMA f (diluted 1:1,000). Secondary antibodies (biotinylated anti-mouse IgG, g anti-rabbit IgG, g or anti-goat IgG g [each diluted 1:200]) were applied to the sections for 30 minutes at 25°C according

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in American Journal of Veterinary Research

protozoa. The observed brain lesions were consistent with severe subacute and chronic DA toxicosis. 7 At the time of death, the otter's serum IgG titer against T gondii was 1:10,240, but disseminated protozoal infection was not histologically observed

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in American Journal of Veterinary Research

positive for CD44 (panel C) and CD90 (panel D). Each MSC sample was incubated with the primary antibody of interest and compared with an unstained sample or a sample incubated with the corresponding isotype (mouse [ms] IgG1-PE for CD34, rat IgG2a

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in American Journal of Veterinary Research

biotinylated erythrocytes developed IgG antibodies against biotinylated erythrocytes, erythrocyte kinetics in these human subjects were not altered. 23 Most dogs that need an erythrocyte transfusion require only short-lived support of the oxygen

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in American Journal of Veterinary Research

with murine MAb anti-human CD61-PE (solid line) or murine IgG-PE isotype control (gray fill). B—Thrombin-stimulated platelets (solid line) and unstimulated platelets (dashed line) labeled with rabbit polyclonal anti-human fibrinogen-FITC (Fib

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in American Journal of Veterinary Research

(final concentration, 2 üg/mL) detected p-selectin (platelet activation) with mouse monoclonal anti-human IgG-PE g (final concentration, 2 üg/mL) as isotype control. 38 Rabbit polyclonal anti-human serotonin-PECy7 h (final concentration, 20 üg

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in American Journal of Veterinary Research

/human/rat CGRP (1:200; slides 5 and 15). f Negative control specimens were incubated with PBS solution. The secondary antibody (1:250) was a goat-raised biotinylated anti-rabbit IgG. g Immunostaining was carried out by use of a commercial kit, h with 3

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in American Journal of Veterinary Research

on the basis of results of an IgG enzyme immunoassay a that was performed on blood obtained from each foal at 24 hours of age. All foals were determined to be healthy on the basis of results of a physical examination, CBC, b and serum biochemical

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in American Journal of Veterinary Research

considered healthy on the basis of results of a complete physical examination and assessment of adequate transfer of passive immunity, which was confirmed by measurement of plasma IgG concentration by use of a commercial immunoassay. a The 2 studies 4

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in American Journal of Veterinary Research

determination and serum biochemical analysis were used before inclusion in the study to confirm their health status. Adequate transfer of passive immunity was confirmed prior to initiation of the study by measurement of plasma IgG concentration with a commercial

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in American Journal of Veterinary Research