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–specific IgG concentration A blood sample (10 mL) was obtained by jugular venipuncture from each of 10 randomly selected calves (3 each from groups 1 and 2 and 4 from group 3) on day 0 and from those same calves on day 8 for determination of serum H somni

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in American Journal of Veterinary Research

IgG ≥ 800 mg/dL) as determined by a commercial assay. a Foals were deemed healthy on the basis of findings on physical examination, CBC, and serum biochemical profile. Each foal was housed in a box stall with its dam and allowed to nurse ad libitum

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in American Journal of Veterinary Research

purified rabbit polyclonal anti–β-actin. q After exposure to each primary antibody, blots were incubated with secondary antibodies (anti-rabbit IgG) conjugated with horseradish peroxidase at a final dilution of 1:1,000 for 1 hour at room temperature and

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in American Journal of Veterinary Research