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administration and are subject to marked variability. 12,29 Platelet function can be assessed in vitro by use of aggregometry with whole blood, platelet-rich plasma, or washed platelets. Light transmission aggregometry following stimulation of platelet-rich

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in American Journal of Veterinary Research

was added; and the platelet-rich plasma was centrifuged at 400 × g for 15 minutes at room temperature. Platelet pellets were then suspended in 10 mL of Tyrode-HEPES (12mM NaHCO 3 , 138mM NaCl, 2.9mM KCl, 10mM HEPES, and 5 μg of PGE 1 /mL; pH, 7

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in American Journal of Veterinary Research

expression . BMC Vet Res . 2020 ; 16 ( 1 ): Article 352 . doi: 10.1186/s12917-020-02566-2 19. Tambella AM , Attili AR , Dupré G , et al . Platelet-rich plasma to treat experimentally-induced skin wounds in animals: a systematic review and meta

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in American Journal of Veterinary Research

LY Mehta P Mehta JL . Platelet inhibitory effect of nitroglycerin in platelet-rich plasma: relevance of glutathione-s-transferases in plasma . J Investig Med 1996 ; 44 : 561 – 565 . 29. Guzey M Vural H Satici A , et al

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in American Journal of Veterinary Research

apoptosis in body and limb wounds in the horse . Wound Repair Regen 2005 ; 13 : 520 – 529 . 10.1111/j.1067-1927.2005.00073.x 17. Monteiro SO Lepage OM Theoret CL . Effects of platelet-rich plasma on the repair of wounds on the distal aspect

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in American Journal of Veterinary Research

protamine causes increased aggregation of platelets and thrombocytopenia in dogs, 6 and results of an in vitro study 7 indicate protamine decreases adenosine diphosphate–induced platelet aggregation. In platelet-rich plasma, the effects of protamine on

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in American Journal of Veterinary Research

thromboxane A2 formation in cat platelet rich plasma . Pharmacol Res Commun 1978 ; 10 : 1 – 12 . 10.1016/S0031-6989(78)80057-5 3. Ting HJ , Murad JP , Espinosa EV , et al. Thromboxane A2 receptor: biology and function of a peculiar receptor

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in American Journal of Veterinary Research

degenerative joint disease. 7 A recheck examination was performed 1 month after surgery. No clinically relevant improvement in lameness was noted. Treatment options were discussed, including corrective osteotomy or intra-articular injection of platelet-rich

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in Journal of the American Veterinary Medical Association

return to normal. Lameness remained static after the treatment, based on owner reports and serial range of motion performed by a rehabilitation specialist. An injection of platelet-rich plasma into the affected right semitendinosus was performed in

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in Journal of the American Veterinary Medical Association

. ABBREVIATIONS COX Cyclooxygenase NSAID Nonsteroidal anti-inflammatory drug TXA 2 Thromboxane A 2 PGE 2 Prostaglandin E 2 PGI 2 Prostacyclin PRP Platelet-rich plasma PPP Platelet-poor plasma TXB 2 Thromboxane B 2 LPS Lipopolysaccharide a

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in American Journal of Veterinary Research