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) and 21 dogs, 4 cats, and 16 humans (part 2), nasal swab specimens from the humans and nasal and rectal swab specimens from the pets were tested for MRSA. In part 1, 6 of 22 (27%) households had a person with MRSA colonization, and in part 2, 1 of 8

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in Journal of the American Veterinary Medical Association

via culture of deep nasal swab samples obtained prior to treatment with tilmicosin (10 mg/kg [4.5 mg/lb], SC). Tilmicosin resistance among the M haemolytica and P multocida isolates was low (6/745 [0.8%] and 16/231 [6.9%], respectively). The

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in Journal of the American Veterinary Medical Association

backgrounding and stocker cattle operations. In a study of 432 calves from 9 such operations, nasal swab specimens were collected from each calf and submitted for bacteriologic culture and PCR assay for M bovis . Ten calves (2%) had positive culture results and

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in Journal of the American Veterinary Medical Association

), they were euthanized immediately. These criteria were consistent with Canadian Council of Animal Care guidelines and were approved by the Committee on Animal Care and Supply at the University of Saskatchewan. Sample collection —Deep nasal swab

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in Journal of the American Veterinary Medical Association

the Canadian Council of Animal Care guidelines and were approved by the Committee on Animal Care and Supply at the University of Saskatchewan. Sample collection —Deep nasal swab specimens were taken from both nares prior to challenge and on days 2

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in Journal of the American Veterinary Medical Association

-detection ELISA. 18 , f Blood samples and nasal swab specimens were collected from PI cows on days 90, 120, and 150 for use in virus isolation and virus titration. 19 Genotypic analysis —After viral isolation and biological cloning of viral isolates from the

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in American Journal of Veterinary Research

isolation by use of sterile polyester nasal swabs prior to viral challenge and daily beginning the day after the challenge through to the end of the 14-day challenge phase. Collected nasal swab specimens were frozen in transport medium (α-modified minimal

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in Journal of the American Veterinary Medical Association

—Beginning 2 days before calves were vaccinated (day of vaccination = day 0), rectal temperature and any clinical signs of infection were recorded daily for each calf. In addition, nasal swab specimens were collected from each calf for use in BVDV isolation

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in American Journal of Veterinary Research

samples (100 μL) were inoculated in duplicate onto confluent Madin-Darby bovine kidney cell monolayers in 6-well plates and incubated at 37°C for 72 hours; plaques were then counted to quantitate PFU. Viral DNA in nasal swabs was also determined by qPCR

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in American Journal of Veterinary Research

measurement, ADG was calculated as the weight gained since processing divided by the number of days on feed in the feedlot. From each calf, nasal swab specimens and 2 blood samples (one [6 mL] collected in a tube that contained an anticoagulant and another

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in Journal of the American Veterinary Medical Association