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superior to a parenteral route of vaccination, which stimulates a high systemic IgG response. Bovine ocular IgG arises primarily from plasma, 32 and results of another study 30 indicate that an increase in the systemic IgG response against MbxA results in

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in American Journal of Veterinary Research

Mannheimia haemolytica and Pasteurella multocida , colonize the upper respiratory tract of Holstein calves within the first week of life. 13 Interferon-γ, also known as immune IFN, has potent immunostimulatory activity and can enhance the IgG response in

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in Journal of the American Veterinary Medical Association

immunodiffusion plates consisted of a homogeneous agarose gel containing either anti-canine IgG or anti-canine IgM with preformed circular wells for deposition of patient serum. Standardized quantities of the patient's serum were pipetted into the preformed

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in Journal of the American Veterinary Medical Association

mucosal damage, thereby preventing or minimizing clinical disease. Because administration of adsorbents to foals coincides with the period of passive transfer of colostral antibodies, 31–33 the effect of adsorbents on immunoglobulin (IgG) absorption must

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in American Journal of Veterinary Research

a high CD4+:CD8+ ratio (T-helper:T-cytotoxic ratio), 4–7 whereas nondiseased pericorneal tissue (conjunctiva and lacrimal gland) is populated predominantly by T-cytotoxic cells. 8 In addition to T cells, antibodies (IgG, IgM, or IgA) cause local

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in American Journal of Veterinary Research

, biochemical, coagulative profile, electrophoresis of serum proteins, and urinalysis did not show any relevant alteration. The serological test for anti-gliadin immunoglobulin G (AG IgG) and anti-transglutaminase-2 immunoglobulin A (ATG-2 IgA) antibodies

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in Journal of the American Veterinary Medical Association

detect inflammatory processes and responses to infection. 19 Infection is often reflected as a decrease in the A/G ratio attributable to increases in concentrations of globulins, including the γ-globulin fraction that contains IgG. An example of a

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in American Journal of Veterinary Research

prepared and evaluated for agglutination. In the indirect immunofluorescence test, 5 paraformaldehyde-fixed neutrophils were incubated with serum from neutropenic cats and subsequently incubated with fluorescein-labeled anti-cat IgG. Cells were viewed with

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in American Journal of Veterinary Research

Animals. The IgG fraction was isolated by use of protein G affinity chromatography d performed in accordance with the manufacturer's protocol; the IgG fraction then was biotinylated as described elsewhere. 17 The purified IgG was absorbed for 60

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in American Journal of Veterinary Research

Goat kids depend on the passive transfer of colostral IgG to provide humoral immunity during the neonatal period, 1-4 and adequate passive transfer of immunity, determined by measurement of serum IgG concentration, is a critical determinant of

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in Journal of the American Veterinary Medical Association