Search Results

You are looking at 11 - 20 of 193 items for :

  • Refine by Access: All Content x
Clear All

. Determinations of plasma cytokine concentrations —Concentrations of proinflammatory (IL-1β, TNF-α, IL-6, and IL-17A) and anti-inflammatory (IL-10 and IL-13) cytokines were measured in plasma samples with specific ELISA commercial kits a according to the

Full access
in American Journal of Veterinary Research

inactivation at 95°C for 5 minutes, the reaction volume was adjusted to 100 μL with nuclease-free water. The cDNA was analyzed immediately or stored at −20°C until use. Real-time PCR systems for equine GAPDH, TNF-α, IFN-γ, IL-2, IL-6, IL-8, IL-10 and TGF

Full access
in American Journal of Veterinary Research

and inhibited organism killing and phagosome acidification. Alternatively, MAPK JNK induced expression of TNF-α and prevented killing of MAP organisms. 11 Overall, results of these studies indicate that the MAPK p38 and MAPK JNK signaling pathways

Full access
in American Journal of Veterinary Research

diagnostic tests, it is worthwhile to evaluate the use of inflammatory mediators as potential biomarkers. In humans with asthma, increases in concentrations of IL-4, TNF-α, and NO metabolites or exhaled NO have been found, prompting interest into further

Full access
in American Journal of Veterinary Research

by triggering the production and release of endogenous mediators of inflammation, including the secretion of cytokines such as TNF-α and IL-6. 13–15 Nearly all LPS effects are strictly dose related. 16 Study of the physiologic effects of LPS

Full access
in American Journal of Veterinary Research

molecular markers (TNF-α, IL-1β, IL-6, IL-8, IL-10, PCT, and TGF-β) in the blood of healthy and sick foals by use of realtime PCR assays. Materials and Methods Case selection —Forty-nine client-owned foals evaluated at the VMTH at the University of

Full access
in American Journal of Veterinary Research

rates. 6–8 Inflammation associated with transfusion results, in part, from the accumulation of cytokines in stored blood products. 1,2 Interleukin-1β, IL-8, and TNF-α are cytokines that increase in concentration in human whole blood and PRBCs during

Full access
in American Journal of Veterinary Research

osteoarthritic process, induces the production of ROS in several cell types. 6–11 Activation with IL-1β or TNF-α increases the production of inducible nitric oxide synthase, which regulates the production of nitric oxide, by chondrocytes in vitro. 9 Excessive

Full access
in American Journal of Veterinary Research

before in horses with EIPH, information exists on increased mRNA expression of IL-1β, IL-8, and TNF-α in the bronchoalveolar lavage fluid of horses with recurrent airway obstruction 8 ; increased mucosal IL-4 and IL-10 expression associated with

Full access
in American Journal of Veterinary Research

-phase proteins in horses. 1 Although many cytokines contribute to the pathogenesis of endotoxic shock, TNF-α is one of the principle mediators of the effects of LPS. It orchestrates a number of inflammatory responses and initiates formation of other molecules

Full access
in American Journal of Veterinary Research