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and placed in sterile saline (0.9% NaCl) solution with gentamicin sulfate (50 mg/L) and amphotericin B (250 μg/mL). Cartilage samples were transported chilled (approx 5°C) to the laboratory. Isolation, expansion, and 3-D culture of chondrocytes were

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in American Journal of Veterinary Research

cells were evaluated for performance in 2-D cultures and for differentiation capacity in 3-D cultures. Cells were seeded on polyester membranes coated with rat tail collagen type I. Cultures were incubated at the air-liquid interface to achieve

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in American Journal of Veterinary Research

difficult. Initial cell numbers, growth factors, time in culture, and plating techniques differ among studies. 21,26,28–30,45,63 A 3-D culture system for chondrogenesis is superior to the monolayer technique. 64 Micromass culture induces a larger amount of

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in American Journal of Veterinary Research