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Summary

A murine hybridoma monoclonal antibody (mab), IBF9, was generated by fusing myeloma cells (P3X63Ag8.653) with spleen cells from a BALB/c mouse immunized with the canine melanoma cell line CML-10c7. Initial screening of hybridoma antibodies was performed by use of an indirect immunoperoxidase assay on formalin-fixed CML- 10c7 cells. The isotype of mab IBF9 was IgGl as determined by radial gel immunodiffusion. The antibody was tested for reactivity against a panel of formalin-fixed, paraffin-embedded normal and neoplastic canine tissues, using immunoperoxidase staining. Immunostaining was observed in melanomas (24 of 38), a few carcinomas, basal cell tumors, and cutaneous lymphosarcomas. Immunostaining was not observed in fibrosarcomas, hemangiosarcomas, hemangiopericytomas, or histiocytomas. Staining of normal adult canine tissues was limited to a few epithelial tissues and a small percentage of lymphocytes. Fetal tissues were not reactive with mab IBF9. There were statistically significant differences in frequency of reactivity among melanomas with regard to oral vs nonoral, malignant vs benign, and mitotic indices ≥ 1 vs mitotic indices < 1. Differences were not significant when tumors were compared for degree of pigmentation or histologic type.

On the basis of these findings, we suggest that mab IBF9 may be of assistance in diagnosis of nonpigmented melanomas and in assessing the malignant potential of melanomas.

Free access
in American Journal of Veterinary Research

Summary

Anecdotal descriptions of atypical FeLV infections, wherein standard clinical elisa or immunofluorescence testing fails to detect active infections, suggest that an unknown proportion of FeLV-infected cats may go undetected. In this study, 127 viremic and nonviremic cats experimentally inoculated with FeLV were evaluated at necropsy for atypical expression of FeLV antigen. Results from viremic cats were in accordance with results of earlier studies on the pathogenesis of FeLV infection in cats, wherein antigen was found in lymphoid and epithelial tissues. Differences in time course or tissue distribution of viral antigen in some cats appeared to be attributable to the challenge virus preparations, consisting of cell-free tumor homogenate or infectious plasma. It was discovered that 5 of 19 of the FeLV challenge-exposed cats that were nonviremic had FeLV-specific antigens in select tissues (bone marrow, spleen, lymph node, and small intestine) 6 to 75 weeks after inoculation. These results indicated an additional category of possible outcomes for cats exposed to FeLV. Localized FeLV infection, as described here, may explain the discordance between clinical disease and laboratory testing for FeLV.

Free access
in American Journal of Veterinary Research

SUMMARY

Effects of immunosuppression were compared in newly hatched chickens given cyclophosphamide (cy) after inoculation with avian nephritis virus (anv). All cy-treated infected chickens died within 13 days after inoculation of the virus and had heavy urate deposits throughout the body. However, non-cy-treated infected, cy-treated noninfected, and non-cy-treated noninfected control chickens survived through the observation period. In a chronologic study, the value of serum uric acid in cy-treated infected chickens was more than 3 times higher than that in non-cy-treated infected chickens, and more than 9 times higher than in noninfected chickens. Serum uric acid values were coincident with the positive degree of anv antigen in the tubular epithelial cells in the kidneys and with the severity of renal degeneration. Serologic and immunohistologic examinations did not reveal detectable antibody and IgG- and IgM-containing cells in the spleen and kidneys of cy-treated infected chickens. However, non-cy-treated infected chickens had an increased number of IgM- and IgG-containing cells and antibody against anv on postinoculation day 6. These findings demonstrated that cy treatment enhanced the susceptibility of chickens to anv infection.

Free access
in American Journal of Veterinary Research

Summary

The polypeptides of serologically related viruses of hemorrhagic enteritis (he) in turkeys, marble spleen disease (msd) in pheasants, and splenomegaly in chickens (smc) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and analyzed by protein immunoblotting with polyclonal antibodies to he virus (hev). The viral polypeptides II, III, IV, V, VI, and VII were detected on sds-page with the size range from 18 to 97 kDa in hev. Viral polypeptides II, III, V, VI, and VII were detected in msd virus and virus of smc. Protein immunoblotting of viral proteins with anti-hev serum revealed antigenic differences between the 3 viruses of avian adenovirus type-II examined. The differences were that the polypeptides II, III, IV, V, VI, and VII were identified in hev and the polypeptides II, V, VI, and VII were identified in msd virus and virus of smc. The bands of penton base (polypeptide III) and fiber (polypeptide IV) were seen in hev only by protein immunoblotting.

Free access
in American Journal of Veterinary Research

SUMMARY

Laboratory raised white-footed mice (Peromyscus leucopus) were inoculated experimentally with live spirochetes (Borrelia burgdorferi), the etiologic agent of Lyme disease (borreliosis). Prior to inoculation, mouse sera were tested with an indirect fluorescent antibody test, and all mice were seronegative. All inoculated mice seroconverted. In tick transmission studies, immature stages of Ixodes dammini and Dermacentor variabilis attached and fed to repletion on mice, but only I dammini transferred spirochetes to uninfected mice. Mice were susceptible to oral infection and transmitted infection to each other through direct contact. Infection did not affect reproduction or development of young born from infected dams, nor did spirochetes appear in the tissues of neonates. Borrelia burgdorferi spirochetes were identified in the kidneys, liver, and spleen of infected mice by the use of the modified Steiner silver stain and a tissue indirect fluorescent antibody test. Spirochetes also were isolated in culture of the heart blood of 1 mouse. Regardless of the source of infection, no mice developed clinical signs or had any pathologic change resulting from infection. Spirochetes were always observed extracellularly within interstitial spaces. Antibody titers persisted for over 4 months in some mice and spirochetes were found in the tissues of 1 mouse that had been infected 1 year earlier.

Free access
in American Journal of Veterinary Research

-needle aspirates of the spleen by comparing those results with histologic diagnoses. Additional information about underlying disease and ultrasonographic appearance was also obtained. Criteria for Selection of Cases Medical records from 247 dogs and cats in

Full access
in Journal of the American Veterinary Medical Association

the esophageal wall was thickened ( Figure 1 ). The spleen was markedly enlarged (weight, 20 g [approx 3% of the ferret's body weight; normal spleen weight is 0.5% to 1.3% of a ferret's body weight]), had rounded edges, and was soft, pale, and mottled

Full access
in Journal of the American Veterinary Medical Association

abnormalities except mild enlargement of the spleen. A nasogastric tube was passed and no net reflux was obtained, but the gastric contents were mixed with a small amount of blood. No current signs of colic were observed. Initial bloodwork revealed a PCV of 32

Free access
in Journal of the American Veterinary Medical Association

, and others are displaced caudally. On the ventrodorsal view, the mass is on the left side of the abdomen, partially overlying the left kidney. According to the location of the mass within the abdomen, the organs potentially involved may be the spleen

Full access
in Journal of the American Veterinary Medical Association

, and hypersplenism. 1,2 The effects of drugs on splenic size have long been recognized. The effects of barbiturates on the spleen were first reported in dogs. 3 In that study, splenic size was subjectively evaluated on abdominal radiographs, which

Full access
in American Journal of Veterinary Research