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- Author or Editor: Jorge Hernandez x
- Immunology x
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Summary
An elisa was compared with the plaque-reduction serum neutralization (prsn) test, for detection of vesicular stomatitis virus (vsv) antibodies in cattle in a vesicular stomatitis enzootic region of Mexico. A total of 325 bovine serum samples were screened for vsv antibodies. The prsn test was performed, using Vero cells. The elisa contained gradient-purified vsv Indiana (Lab strain) and vsv New Jersey (Hazelhurst) as the antigens. Regression analysis and weighted kappa statistic were used to estimate measures of agreement between the 2 assays for detection of vsv antibodies. The elisa method proved useful for sero-diagnosis of vesicular stomatitis The elisa and prsn test results were highly correlated for detection of vsv antibodies.
