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Abstract

Objective

To investigate the effect of water temperature on hematologic and biochemical analytes in hybrid striped bass.

Animals

Hybrid striped bass (reciprocal cross; female Morone chrysops × male M saxatilis) maintained in 2,000-L tanks with undergravel filters.

Procedure

Fish were acclimated to 10. 18, 24, and 29 C water for 6 weeks prior to sample collection. Hematologic and serum biochemical profiles were then determined. Values were compared among the various temperatures, and with reference intervals previously determined.

Results

Most values were within or slightly outside the established reference intervals. The following analytes deviated notably from the reference interval: leukocyte, lymphocyte, and monocyte counts were lower than the reference intervals at 10 C; glucose values were lower at 10 and 18 C; calcium values were higher at 10 and 18 C; and total protein, albumin, globulin, and chloride values were higher at 29 C.

Conclusion

Separate reference intervals should be developed for analytes which, because of temperature, deviate notably from the reference interval. Modifications of the established reference intervals, by including fish from varied temperatures, should allow use of one reference interval for analytes, with only slight variation attributable to temperature.

Clinical Relevance

Determining the effects of temperature on the hematologic and biochemical values helps develop clinical pathology as a diagnostic tool in fish. (Am J Vet Res 1997;58:126–130)

Free access
in American Journal of Veterinary Research

Abstract

Objective

To investigate the effects of poor water quality on hematologic and biochemical analytes in hybrid striped bass.

Animals

Hybrid striped bass (reciprocal cross: female Morone chrysops × male M saxatilis) maintained in 2,000-L tanks with undergravel filters.

Procedure

Fish were acclimated to high ammonia (0.15 mg/L) and nitrate (200 mg/L) concentrations for 6 weeks prior to sample collection. Hematologic and biochemical profiles were determined for these fish and for fish kept under normal conditions (control). Comparisons were made among the 3 water qualities and with reference intervals determined previously.

Results

Significant differences in hematologic and biochemical analytes were observed between fish in the various groups; however, most of the values were within established reference intervals. All values from fish in the high ammonia concentration tank were either within the reference interval or not significantly different from control values. Fish from the high nitrate concentration tank had higher serum creatinine values and lower chloride values than did control fish, and both analytes were substantially outside the reference intervals.

Conclusion

High ammonia concentration of 0.15 mg/L did not affect any of the blood analytes measured. The hypercreatininemia and hypochloremia observed in fish from the 200 mg of nitrate/ml tank were considered to be pathologic changes associated with the high nitrate concentration.

Clinical Relevance

Determining the effects of water quality on hematologic and biochemical values helps to develop clinical pathology as a diagnostic tool in fish. (Am J Vet Res 1997;58:131–135)

Free access
in American Journal of Veterinary Research

Abstract

Objective

To determine and compare biochemical reference intervals for sunshine bass (hybrid striped bass) from 3 culture systems.

Design

Observational comparison study.

Animals

Clinically normal sunshine bass (reciprocal hybrid striped bass, female Morone chrysops × male M saxatilis) raised in high-density recirculating systems (80 g/L), low-density tanks (5 g/L) with biofilters, and cages (70 g/L) in a fresh water pond.

Procedure

Biochemical reference intervals were determined for fish from the different production systems following the National Committee for Clinical Laboratory Standards guidelines. Reference intervals from the 3 groups were compared.

Results

Reference intervals were significantly different between sunshine bass in the various culture systems. Though most of the differences in reference intervals were minor, fish in the high-density recirculating system had higher concentrations of total protein, albumin, globulin, creatinine, and phosphorus, and lower chloride values. There were no significant differences in glucose concentrations among the 3 groups of fish, and no differences in cortisol concentrations between fish in tanks and cages.

Conclusions

Separate reference intervals should be developed for hybrid striped bass in different culture systems.

Clinical Relevance

Determining biochemical reference intervals for hybrid striped bass provides a tool to assess the health status of these fish. (Am J Vet Res 1996; 57:624–627)

Free access
in American Journal of Veterinary Research

Abstract

Objective

To determine and compare hematologic reference intervals for hybrid striped bass from different culture systems and for 2 types of hybrid.

Design

Observational comparison study.

Animals

Clinically normal sunshine bass (reciprocal hybrid striped bass, female Morone chrysops × male M saxatilis) raised in high-density recirculating systems, sunshine bass raised in tanks with biofilters, and palmetto bass (original hybrid striped bass, female M saxatilis × male M chrysops) raised in tanks with biofilters.

Procedure

Hematologic reference intervals were determined for fish from the different production systems, following the National Committee for Clinical Laboratory Standards guidelines. Reference intervals from the 3 groups were compared.

Results

Reference intervals were significantly different between sunshine bass in the 2 culture systems and between sunshine and palmetto bass. Many of the differences were slight, but notable differences were observed. Sunshine bass in recirculating systems had higher total leukocyte, lymphocyte, neutrophil, and monocyte counts than did either hybrid in tanks. Palmetto bass had a greater number of neutrophils than monocytes, whereas sunshine bass had more monocytes than neutrophils. Additionally, palmetto bass had a lower PCV and hemoglobin value than did either group of sunshine bass.

Conclusions

Separate reference intervals should be developed for hybrid striped bass in different culture systems and for different types of hybrids.

Clinical Relevance

Determining reference intervals for hybrid striped bass provides a tool to assess the health status of these fish. (Am J Vet Res 1996; 57:618–623)

Free access
in American Journal of Veterinary Research