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  • Author or Editor: Gary J. Kociba x
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SUMMARY

Chemotaxis under agarose was evaluated to establish an assay system and to characterize chemotactic responses of canine neutrophils. A method for the measurement of canine neutrophil chemotaxis was established, with optimal responses obtained with agarose containing 10% pooled canine serum, a concentration of 5 × 105 cells/well, zymosan-activated serum (zas), or autologous serum or plasma as the chemoattractants, and a 120-minute incubation period. Canine neutrophils responded well to zas, heat-inactivated zas, autologous serum and plasma, and heat-inactivated pooled serum. Chemotactic activity was proportional to the concentration of serum used as the chemoattractant. Mean (± sd) random migration, chemotaxis, chemotactic index, and chemotactic differential of neutrophils from 9 healthy Greyhounds were 1.09 (± 0.23), 1.95 (± 0.38), 1.82 (± 0.31), and 0.86 (± 0.32) mm, respectively.

Free access
in American Journal of Veterinary Research

Summary

Leukocyte adhesion deficiency was diagnosed in 4 Holstein calves from 1 to 4 months old. Calves had severe ulcers on oral mucous membranes, gingivitis, severe periodontitis, chronic pneumonia, and stunted growth associated with severe neutrophilia. Neutrophils from affected calves had function defect, characterized by severely decreased adherence, chemotactic movements, phagocytosis, luminol-dependent chemiluminescent response, and O- 2-producing activities. Deficient CD18 expression (0.1 to 1.7%) on neutrophils was clearly detected by use of flow cytometric analysis. These affected calves were linked to a common ancestral sire that has been documented to be a carrier. Clinical features, leukocyte functional abnormalities, deficient expression of CD18, and mode of inheritance indicated that affected calves had leukocyte adhesion deficiency. In vitro leukocyte functional abnormalities were associated with deficiency in the expression of CD11/CD18. Pathologic findings indicated possible increased susceptibility to infection associated with this disease.

Free access
in American Journal of Veterinary Research

Abstract

Objective

To determine reference ranges for hematologic and serum biochemical variables of bulls residing at an artificial insemination center.

Animals

225 healthy Holstein bulls categorized by age into yearling, intermediate age, and adult groups.

Procedure

Hematologic and serum biochemical analyses were performed on 1 blood and 1 serum sample from each bull.

Results

Significant differences associated with age were identified for 25 of 33 variables. Serum creatinine concentration for clinically normal adult bulls (2.44 ± 0.33 mg/dl) was higher than previously reported reference values for adult cattle. There was a reversal of the segmented neutrophil-to-lymphocyte ratio between yearling (0.85:1) and adult (2.6:1) bulls. This was associated with a significant and marked decrease in absolute numbers of lymphocytes per microliter between yearling (5,801 ± 1,683) and adult (1,307 ± 509) bulls.

Clinical Relevance

Reference values for selected clinicopathologic variables were generated from the data. (Am J Vet Res 1998;59:1386–1391)

Free access
in American Journal of Veterinary Research

SUMMARY

Marked differences in bone marrow cellularity were observed between cattle affected with leukocyte adhesion deficiency (lad) and control cattle. The number of nucleated cells in bone marrow was 2.9 to 8.8 times higher in cattle affected with lad, compared with controls. The myeloid-to-erythroid ratio of bone marrow from 3 cattle affected with lad ranged from 2.4 to 12. Deficient CD18 expression on neutrophils isolated from bone marrow of cattle with lad was clearly detected by flow cytometric analysis. Neutrophils from bone marrow of cattle affected with lad appeared round and not flat, after adherence to plastic wells under agarose, whereas neutrophils from bone marrow of clinically normal cattle were firmly spread on the surface of plastic wells. In the chemotaxis under-agarose assay, many pseudopodia were detected on bone marrow neutrophils from clinically normal cattle, but were not detected on bone marrow neutrophils from cattle with lad. Activities of chemotactic movements and phagocytosis of neutrophils isolated from bone marrow of cattle affected with lad were documented to be severely impaired.

Free access
in American Journal of Veterinary Research