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  • Author or Editor: Everett C. Short Jr. x
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Cell extracts that were prepared from blood mononuclear leukocytes from 66 samples obtained from 6 clinically normal calves contained mean 2′,5′-oligoadenylate (2′,5′-oligo[A]) synthetase activity sufficient to synthesize 186 ± 82 pmol of 2′,5′-oligo(A)/h/106 cells. Calves had no measurable serum interferon (ifn) activity. Five calves were given im injections of 104, 105, 5 × 105, 106, and 107 U of bovine ifn-α1/kg of body weight at 2-week intervals. Five dosing sequences were used with a 5 × 5 Latin square design so that each calf received each dose once. Activity of 2′,5′-oligo(A) synthetase increased at 24 hours in response to all dosages of ifn and then declined following first-order kinetics, with an apparent half-life (t½) of 2.1 ± 0.5 days. The area under the concentration-time curve for 2′,5′-oligo(A) synthetase increased with dose of ifn more rapidly than did peak response. Serum ifn that was measured at 1-day intervals following administration of ifn was consistently measurable only at dosages above 106 U of ifn/kg. The t½ for circulating ifn was 12.4 ± 1.0 hours. Over all dosages, increases in 2′,5′-oligo(A) synthetase activity were measurable for 3.5 days longer than were increases in ifn following im injection of ifn. None of the calves developed detectable anti-ifn antibodies.

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in American Journal of Veterinary Research