Objective—To describe the kinetics of urinary recovery
(UR) of 5 sugars used for gastrointestinal permeability
and mucosal function testing following orogastric
administration of lactose, rhamnose, xylose,
methylglucose, and sucrose.
Animals—7 healthy male Beagles.
Procedure—A sugar solution containing lactulose,
rhamnose, xylose, methylglucose, and sucrose was
administered by orogastric intubation to healthy dogs.
Urine samples were collected immediately before
sugar solution administration (baseline) and at 2-hour
intervals thereafter. The UR of the 5 sugars was determined
from urine concentrations measured by high
pressure liquid chromatography and pulsed amperometric
detection. Percent urinary recovery (%UR) of the
total UR up to 12 hours after sugar solution administration
was calculated for each sugar at 2-hour intervals.
Results—Mean %UR exceeded 85% for all 5 sugars
at 6 hours after orogastric administration of the sugar
solution and exceeded 90% after 8 hours.
Conclusion and Clinical Relevance—In healthy
dogs, a urine collection period of 6 hours is sufficient
for gastrointestinal permeability and mucosal function
testing following orogastric administration of lactulose,
rhamnose, xylose, methylglucose, and sucrose.
(Am J Vet Res 2002;63:845–848)
Objective—To develop and validate an ELISA for
quantitative analysis of feline trypsin-like immunoreactivity
Sample Population—Purified feline cationic trypsin
(fCT) and rabbit anti-fCT antiserum; blood samples
from 63 healthy cats.
Procedures—A sandwich capture ELISA was developed,
using anti-fCT antiserum purified by affinity
chromatography that underwent biotinylation.
Purified fCT was used for standards. The assay was
validated by determination of sensitivity, working
range, linearity, accuracy, precision, and reproducibility.
A reference range was established by assaying
serum samples from the 63 healthy cats.
Results—Sensitivity was 1.23 µg/L; working range
was 2 to 567 µg/L. Ratios of observed versus
expected results for 4 samples tested at various
dilutions ranged from 90.0 to 120.7%. Ratios of
observed versus expected results for 5 samples
spiked with various concentrations of fCT ranged
from 82.0 to 101.8%. Intra- and inter-assay coefficients
of variability ranged from 9.9 to 11.1% and
from 10.2 to 21.7%, respectively. The reference
range for serum fTLI measured with this ELISA was
12 to 82 µg/L.
Conclusions and Clinical Relevance—Results suggest
that an ELISA can be used to measure serum
fTLI in cats. The ELISA was sufficiently sensitive, linear,
accurate, precise, and reproducible for clinical
use. (Am J Vet Res 2000;61:620–623)