Citrate phosphate dextrose adenine (CPDA-1) can be sampled from blood collection bags in a multi-dose manner

Cheryl M. Stout Department of Avian and Exotics, Red Bank Veterinary Hospital, Tinton Falls, NJ

Search for other papers by Cheryl M. Stout in
Current site
Google Scholar
PubMed
Close
 DVM
,
Jessie M. Campanile Wyatt Goldthwaite Veterinary Blood Bank, Red Bank Veterinary Hospital, Tinton Falls, NJ

Search for other papers by Jessie M. Campanile in
Current site
Google Scholar
PubMed
Close
 BS
, and
S. Emmanuelle Knafo Department of Avian and Exotics, Red Bank Veterinary Hospital, Tinton Falls, NJ

Search for other papers by S. Emmanuelle Knafo in
Current site
Google Scholar
PubMed
Close
 DVM, DACZM

Abstract

OBJECTIVE

To evaluate the sterility of citrate phosphate dextrose adenine (CPDA-1) anticoagulant when sampled from blood collection bags in a multi-dose manner.

SAMPLE

10 pre-filled CPDA-1 blood collection bags; 46 bacterial and 28 fungal culture result reports.

PROCEDURES

10 CPDA-1 blood collection bags were split into 2 equal groups and stored at either room temperature (24 °C) or refrigerator temperature (5 °C) for 30 days. Two bags in each group were designated as controls. Beginning on day 0 a 1.0 mL aliquot was withdrawn from each experimental bag and submitted for bacterial culture (aerobic and anaerobic) every 5 days, and fungal culture every 10 days. All 10 bags were sampled on day 30. Bacterial and fungal culture results were compiled and interpreted.

RESULTS

46 CPDA-1 aliquots were cultured, resulting in 2 positive microbial isolates: Bacillus was cultured from a previously unopened experimental bag on day 0, and Candida was cultured from a refrigerated experimental bag on day 30. Both positives are thought to represent post-sampling contamination, though these suspicions cannot be confirmed in the bag yielding Candida due to a lack of subsequent data. All other samples were negative for microbial growth.

CLINICAL RELEVANCE

CPDA-1 blood collection bags stored at either 24 °C or 5 °C can be used in a multi-dose manner for up to 20 days when each sample is obtained aseptically. These results support the clinician’s ability to utilize the contents of 1 bag multiple times rather than discarding the bag after a single use.

Abstract

OBJECTIVE

To evaluate the sterility of citrate phosphate dextrose adenine (CPDA-1) anticoagulant when sampled from blood collection bags in a multi-dose manner.

SAMPLE

10 pre-filled CPDA-1 blood collection bags; 46 bacterial and 28 fungal culture result reports.

PROCEDURES

10 CPDA-1 blood collection bags were split into 2 equal groups and stored at either room temperature (24 °C) or refrigerator temperature (5 °C) for 30 days. Two bags in each group were designated as controls. Beginning on day 0 a 1.0 mL aliquot was withdrawn from each experimental bag and submitted for bacterial culture (aerobic and anaerobic) every 5 days, and fungal culture every 10 days. All 10 bags were sampled on day 30. Bacterial and fungal culture results were compiled and interpreted.

RESULTS

46 CPDA-1 aliquots were cultured, resulting in 2 positive microbial isolates: Bacillus was cultured from a previously unopened experimental bag on day 0, and Candida was cultured from a refrigerated experimental bag on day 30. Both positives are thought to represent post-sampling contamination, though these suspicions cannot be confirmed in the bag yielding Candida due to a lack of subsequent data. All other samples were negative for microbial growth.

CLINICAL RELEVANCE

CPDA-1 blood collection bags stored at either 24 °C or 5 °C can be used in a multi-dose manner for up to 20 days when each sample is obtained aseptically. These results support the clinician’s ability to utilize the contents of 1 bag multiple times rather than discarding the bag after a single use.

All Time Past Year Past 30 Days
Abstract Views 619 6 0
Full Text Views 1856 1590 176
PDF Downloads 1393 1114 132
Advertisement